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Communication

Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes

1
Centro de Química de Vila Real (CQ-VR), Universidade de Trás-os-Montes e Alto Douro (UTAD), 5000-801 Vila Real, Portugal
2
Departamento de Agronomia, Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
3
Centro de Biologia Molecular e Ambiental (CBMA), Department of Biology, UMINHO, 4710-057 Braga, Portugal
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Centro de Investigação e Tecnologias Agro-Ambientais e Biológicas (CITAB), Universidade de Trás-os-Montes e Alto Douro (UTAD), Quinta de Prados, 5000-801 Vila Real, Portugal
5
CoLAB Vines&Wines, Associação para o Desenvolvimento da Viticultura Duriense (ADVID), Régia Douro Park, 5000-033 Vila Real, Portugal
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 306; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010306
Received: 7 November 2019 / Revised: 24 December 2019 / Accepted: 31 December 2019 / Published: 2 January 2020
(This article belongs to the Special Issue Plant Elicitors of Resistance and the Future of Plant Protection)
Despite the numerous beneficial properties and uses of chitosan in agriculture, the molecular mechanisms behind its elicitation potential are still unclear. This study aimed at understanding the effect of chitosan application in the levels of phenolic compounds of Vitis vinifera L. red grapes berry skin (cv. Tinto Cão) during veraison. Grapevines were treated with chitosan (0.01% in 0.01% acetic acid) while control grapevines were sprayed with 0.01% acetic acid. Results showed that several monomeric anthocyanins increased significantly in berry skins after treatment with chitosan. Additionally, Catechin, Rutin and Querecetin-3-O-galactoside were also recorded in higher amount upon chitosan treatment. Besides modulating the phenolic content, chitosan treatment also induced modifications in several target genes encoding key enzymes and transporters involved in secondary metabolic pathways. For instance, the genes PAL, CHS, F3H, ANR, UFGT, ABCC1, GST, MATE1 were upregulated in leaves and berry skins at veraison cessation in response to chitosan treatment. Overall, the results demonstrated that chitosan has a stimulatory effect on the accumulation of phenolic compounds, including anthocyanins, mediated by modifications in the transcription of key genes involved in their biosynthesis and transport in grape berries. View Full-Text
Keywords: chitosan; elicitor; Vitis vinifera L.; anthocyanins; polyphenols; tannins; transporter genes chitosan; elicitor; Vitis vinifera L.; anthocyanins; polyphenols; tannins; transporter genes
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MDPI and ACS Style

Singh, R.K.; Martins, V.; Soares, B.; Castro, I.; Falco, V. Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes. Int. J. Mol. Sci. 2020, 21, 306. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010306

AMA Style

Singh RK, Martins V, Soares B, Castro I, Falco V. Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes. International Journal of Molecular Sciences. 2020; 21(1):306. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010306

Chicago/Turabian Style

Singh, Rupesh K., Viviana Martins, Bruno Soares, Isaura Castro, and Virgílio Falco. 2020. "Chitosan Application in Vineyards (Vitis vinifera L. cv. Tinto Cão) Induces Accumulation of Anthocyanins and Other Phenolics in Berries, Mediated by Modifications in the Transcription of Secondary Metabolism Genes" International Journal of Molecular Sciences 21, no. 1: 306. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21010306

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