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Article

A Phytochemical Constituent, (E)-Methyl-Cinnamate Isolated from Alpinia katsumadai Hayata Suppresses Cell Survival, Migration, and Differentiation in Pre-Osteoblasts

1
Department of Oral and Maxillofacial Pathology, School of Dentistry, Kyung Hee University, Seoul 02453, Korea
2
National Development Institute of Korean Medicine, Gyeongsan 38540, Korea
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(10), 3700; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103700
Received: 15 April 2020 / Revised: 16 May 2020 / Accepted: 19 May 2020 / Published: 24 May 2020
(This article belongs to the Special Issue Novel Extracellular and Intracellular Signalling Mechanisms in Bone)
Background: (E)-methyl-cinnamate (EMC), a phytochemical constituent isolated from Alpinia katsumadai Hayata, is a natural flavor compound with anti-inflammatory properties, which is widely used in the food and commodity industry. However, the pharmacological effects of methyl-cinnamate on pre-osteoblasts remain unknown. This study aimed to investigate the pharmacological effects and mechanisms of EMC in pre-osteoblast MC3T3-E1 cells (pre-osteoblasts). Methods: Cell viability and apoptosis were evaluated using the MTT assay and TUNEL staining. Cell migration and osteoblast differentiation were examined using migration assays, as well as alkaline phosphatase activity and staining assays. Western blot analysis was used to examine intracellular signaling pathways and apoptotic proteins. Results: EMC decreased cell viability with morphological changes and increased apoptosis in pre-osteoblasts. EMC also induced the cleavage of Poly (ADP-ribose) polymerase (PARP) and caspase-3 and reduced the expression of anti-apoptotic proteins. In addition, EMC increased TUNEL-positive cells in pre-osteoblasts, decreased the activation of mitogen-activated protein kinases, and suppressed cell migration rate in pre-osteoblasts. Subsequently, EMC inhibited the osteoblast differentiation of pre-osteoblasts, as assessed by alkaline phosphatase staining and activity assays. Conclusion: These findings demonstrate that EMC has a pharmacological and biological role in cell survival, migration, and osteoblast differentiation. It suggests that EMC might be a potential phytomedicine for treating abnormalities of osteoblast function in bone diseases. View Full-Text
Keywords: phytomedicine; Alpinia katsumadai Hayata; (E)-methyl-cinnamate; osteoblast; apoptosis; MAPKs phytomedicine; Alpinia katsumadai Hayata; (E)-methyl-cinnamate; osteoblast; apoptosis; MAPKs
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MDPI and ACS Style

Park, K.-R.; Lee, H.; Cho, M.; Yun, H.-M. A Phytochemical Constituent, (E)-Methyl-Cinnamate Isolated from Alpinia katsumadai Hayata Suppresses Cell Survival, Migration, and Differentiation in Pre-Osteoblasts. Int. J. Mol. Sci. 2020, 21, 3700. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103700

AMA Style

Park K-R, Lee H, Cho M, Yun H-M. A Phytochemical Constituent, (E)-Methyl-Cinnamate Isolated from Alpinia katsumadai Hayata Suppresses Cell Survival, Migration, and Differentiation in Pre-Osteoblasts. International Journal of Molecular Sciences. 2020; 21(10):3700. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103700

Chicago/Turabian Style

Park, Kyung-Ran, Hanna Lee, MyoungLae Cho, and Hyung-Mun Yun. 2020. "A Phytochemical Constituent, (E)-Methyl-Cinnamate Isolated from Alpinia katsumadai Hayata Suppresses Cell Survival, Migration, and Differentiation in Pre-Osteoblasts" International Journal of Molecular Sciences 21, no. 10: 3700. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103700

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