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Article

Blood Oxidative Stress Modulates Alveolar Bone Loss in Chronically Stressed Rats

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Laboratory of Functional and Structural Biology, Institute of Biological Sciences, Federal University of Para, Belem 66075-900, PA, Brazil
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Laboratory of In Vitro Assays, Immunology and Microbiology, Faculty of Pharmacy, Institute of Biological Sciences, Federal University of Pará, Belem 66075-900, PA, Brazil
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Laboratory of Pharmacology of Inflammation and Behavior, Faculty of Pharmacy, Institute of Health Science, Federal University of Pará, Belem 66075-900, PA, Brazil
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Cancer and Inflammation Research Laboratory, Department of Morphology, Federal University of Rio Grande do Norte, Natal 59078-970, RN, Brazil
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Post-Graduation Programme in Health Science, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil
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Post-Graduation Programme in Structural and Functional Biology, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil
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Department Biophysics and Pharmacology, Post-Graduation Programme in Public Health, Post-Graduation Programme in Pharmaceutical Science, Federal University of Rio Grande do Norte, Natal 59072-970, RN, Brazil
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Dental Materials Laboratory, Faculty of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90040-060, Brazil
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Department of Periodontology, Faculty of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre 90040-060, Brazil
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(10), 3728; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103728
Received: 26 February 2020 / Revised: 16 March 2020 / Accepted: 18 March 2020 / Published: 25 May 2020
(This article belongs to the Special Issue Molecular Mechanisms of Periodontal Disease)
We aimed to investigate the effects of chronic stress (CS) on experimental periodontitis (EP) in rats. For this, 28 Wistar rats were divided into four groups: control, ligature-induced experimental periodontitis (EP), chronic stress (CS; by physical restraint model) and CS+EP (association of chronic stress and ligature-induced periodontitis). The experimental period lasted 30 days, including exposure to CS every day and ligature was performed on the 15th experimental day. After 30 days, the animals were submitted to the behavioral test of the elevated plus maze (EPM). Next, rats were euthanized for blood and mandible collection in order to evaluate the oxidative biochemistry (by nitric oxide (NO), reduced-glutathione activity (GSH), and thiobarbituric acid reactive substance levels (TBARS)) and alveolar bone characterization (by morphometric, micro-CT, and immunohistochemistry), respectively. The behavioral parameters evaluated in EPM indicated higher anxiogenic activity in the CS and CS+EP, groups, which is a behavioral reflex of CS. The results showed that CS was able to change the blood oxidative biochemistry in CS and CS+EP groups, decrease GSH activity in the blood, and increase the NO and TBARS concentrations. Thus, CS induces oxidative blood imbalance, which can potentialize or generate morphological, structural, and metabolic damages to the alveolar bone. View Full-Text
Keywords: chronic stress; experimental periodontitis; alveolar bone loss; ligatures chronic stress; experimental periodontitis; alveolar bone loss; ligatures
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MDPI and ACS Style

Lopes Castro, M.M.; Nascimento, P.C.; Souza-Monteiro, D.; Santos, S.M.; Arouck, M.B.; Garcia, V.B.; Araújo, R.F.d., Jr.; de Araujo, A.A.; Balbinot, G.d.S.; Collares, F.M.; Rosing, C.K.; Monteiro, M.C.; Ferraz Maia, C.S.; Lima, R.R. Blood Oxidative Stress Modulates Alveolar Bone Loss in Chronically Stressed Rats. Int. J. Mol. Sci. 2020, 21, 3728. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103728

AMA Style

Lopes Castro MM, Nascimento PC, Souza-Monteiro D, Santos SM, Arouck MB, Garcia VB, Araújo RFd Jr., de Araujo AA, Balbinot GdS, Collares FM, Rosing CK, Monteiro MC, Ferraz Maia CS, Lima RR. Blood Oxidative Stress Modulates Alveolar Bone Loss in Chronically Stressed Rats. International Journal of Molecular Sciences. 2020; 21(10):3728. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103728

Chicago/Turabian Style

Lopes Castro, Micaele M., Priscila C. Nascimento, Deiweson Souza-Monteiro, Sávio M. Santos, Mayra B. Arouck, Vinicius B. Garcia, Raimundo F.d. Araújo Jr., Aurigena A. de Araujo, Gabriela d.S. Balbinot, Fabrício M. Collares, Cassiano K. Rosing, Marta C. Monteiro, Cristiane S. Ferraz Maia, and Rafael R. Lima 2020. "Blood Oxidative Stress Modulates Alveolar Bone Loss in Chronically Stressed Rats" International Journal of Molecular Sciences 21, no. 10: 3728. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103728

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