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The Separation and Characterization of Extracellular Vesicles from Medium Conditioned by Bovine Embryos
Article

Oviductal Extracellular Vesicles Improve Post-Thaw Sperm Function in Red Wolves and Cheetahs

1
Smithsonian National Zoo and Conservation Biology Institute, 1500 Remount Road, Front Royal, VA 22630, USA
2
The Irving K. Barber School of Arts and Sciences, The University of British Columbia, Okanagan Campus, 1177 Research Road, Kelowna, BC V1V 1V7, Canada
*
Author to whom correspondence should be addressed.
Authors contributed equally to this research.
Int. J. Mol. Sci. 2020, 21(10), 3733; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103733
Received: 28 April 2020 / Revised: 15 May 2020 / Accepted: 20 May 2020 / Published: 25 May 2020
(This article belongs to the Special Issue Extracellular Vesicles in Reproduction)
Artificial insemination (AI) is a valuable tool for ex situ wildlife conservation, allowing the re-infusion and dissemination of genetic material, even after death of the donor. However, the application of AI to species conservation is still limited, due mainly to the poor survival of cryopreserved sperm. Recent work demonstrated that oviductal extracellular vesicles (oEVs) improved cat sperm motility and reduced premature acrosomal exocytosis. Here, we build on these findings by describing the protein content of dog and cat oEVs and investigating whether the incubation of cryopreserved red wolf and cheetah sperm with oEVs during thawing improves sperm function. Both red wolf and cheetah sperm thawed with dog and cat oEVs, respectively, had more intact acrosomes than the non-EV controls. Moreover, red wolf sperm thawed in the presence of dog oEVs better maintained sperm motility over time (>15%) though such an improvement was not observed in cheetah sperm. Our work demonstrates that dog and cat oEVs carry proteins important for sperm function and improve post-thaw motility and/or acrosome integrity of red wolf and cheetah sperm in vitro. The findings show how oEVs can be a valuable tool for improving the success of AI with cryopreserved sperm in threatened species. View Full-Text
Keywords: wildlife; gamete rescue; oviduct; cryopreservation; sperm wildlife; gamete rescue; oviduct; cryopreservation; sperm
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MDPI and ACS Style

de Almeida Monteiro Melo Ferraz, M.; Nagashima, J.B.; Noonan, M.J.; Crosier, A.E.; Songsasen, N. Oviductal Extracellular Vesicles Improve Post-Thaw Sperm Function in Red Wolves and Cheetahs. Int. J. Mol. Sci. 2020, 21, 3733. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103733

AMA Style

de Almeida Monteiro Melo Ferraz M, Nagashima JB, Noonan MJ, Crosier AE, Songsasen N. Oviductal Extracellular Vesicles Improve Post-Thaw Sperm Function in Red Wolves and Cheetahs. International Journal of Molecular Sciences. 2020; 21(10):3733. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103733

Chicago/Turabian Style

de Almeida Monteiro Melo Ferraz, Marcia, Jennifer B. Nagashima, Michael J. Noonan, Adrienne E. Crosier, and Nucharin Songsasen. 2020. "Oviductal Extracellular Vesicles Improve Post-Thaw Sperm Function in Red Wolves and Cheetahs" International Journal of Molecular Sciences 21, no. 10: 3733. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21103733

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