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Article

AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis

1
College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
2
State Key Laboratory of Crop Stress Adaptation and Improvement, School of Life Sciences, Henan University, Kaifeng 475000, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(11), 3991; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21113991
Received: 30 April 2020 / Revised: 26 May 2020 / Accepted: 31 May 2020 / Published: 2 June 2020
(This article belongs to the Special Issue ROS and Abiotic Stress in Plants)
Although most osmotic stresses are harmful to plant growth and development, certain drought- or polyethylene glycol (PEG)-induced mild osmotic stresses promote plant root growth. The underlying regulatory mechanisms of this response remain elusive. Here, we report that the GLYCOGEN SYNTHASE KINASE 3 (GSK3) genes ARABIDOPSIS THALIANA SHAGGY-RELATED KINASE 11 (AtSK11) (AT5G26751) and AtSK12 (AT3G05840) are involved in the mild osmotic stress (−0.4 MPa) response in Arabidopsis thaliana. When grown on plant medium infused with different concentrations of PEG to mimic osmotic stress, both wild-type (WT) and atsk11atsk12 plants showed stimulated root growth under mild osmotic stress (−0.4 MPa) but repressed root growth under relatively strong osmotic stress (−0.5, −0.6, −0.7 MPa) as compared to the mock condition (−0.25 MPa). The root growth stimulation of atsk11atsk12 was more sensitive to −0.4 MPa treatment than was that of WT, indicating that AtSK11 and AtSK12 inhibit the mild stress-induced root growth response. RNA-seq analysis of WT and atsk11atsk12 plants under three water potentials (−0.25 MPa, −0.4 MPa, −0.6 MPa) revealed 10 differentially expressed candidate genes mainly involved in cell wall homeostasis, which were regulated by AtSK11 and AtSK12 to regulate root growth in response to the mild stress condition (−0.4 MPa). Promoter motif and transcription factor binding analyses suggested that the basic helix-loop-helix (bHLH) transcription factor bHLH69/LJRHL1-LIKE 2 (LRL2) may directly regulate the expression of most −0.4 MPa-responsive genes. These findings indicate that mild osmotic stress (−0.4 MPa) promotes plant growth and that the GSK3 family kinase genes AtSK11 and AtSK12 play a negative role in the induction of root growth in response to mild osmotic stress. View Full-Text
Keywords: GSK3; water potential; osmotic stress; root growth; Arabidopsis thaliana GSK3; water potential; osmotic stress; root growth; Arabidopsis thaliana
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MDPI and ACS Style

Dong, L.; Wang, Z.; Liu, J.; Wang, X. AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis. Int. J. Mol. Sci. 2020, 21, 3991. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21113991

AMA Style

Dong L, Wang Z, Liu J, Wang X. AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis. International Journal of Molecular Sciences. 2020; 21(11):3991. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21113991

Chicago/Turabian Style

Dong, Long, Zhixin Wang, Jing Liu, and Xuelu Wang. 2020. "AtSK11 and AtSK12 Mediate the Mild Osmotic Stress-Induced Root Growth Response in Arabidopsis" International Journal of Molecular Sciences 21, no. 11: 3991. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21113991

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