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Review

Genome Editing in Cereals: Approaches, Applications and Challenges

1
Department of Botany, Savitribai Phule Pune University, Pune 411007, India
2
Agri-Biotechnology Division, National Agri-Food Biotechnology Institute (NABI), Mohali 140306, India
3
ICAR-National Rice Research Institute, Cuttack 753006, India
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(11), 4040; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114040
Received: 8 November 2019 / Revised: 18 December 2019 / Accepted: 19 December 2019 / Published: 5 June 2020
(This article belongs to the Section Molecular Biology)
Over the past decades, numerous efforts were made towards the improvement of cereal crops mostly employing traditional or molecular breeding approaches. The current scenario made it possible to efficiently explore molecular understanding by targeting different genes to achieve desirable plants. To provide guaranteed food security for the rising world population particularly under vulnerable climatic condition, development of high yielding stress tolerant crops is needed. In this regard, technologies upgradation in the field of genome editing looks promising. Clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 is a rapidly growing genome editing technique being effectively applied in different organisms, that includes both model and crop plants. In recent times CRISPR/Cas9 is being considered as a technology which revolutionized fundamental as well as applied research in plant breeding. Genome editing using CRISPR/Cas9 system has been successfully demonstrated in many cereal crops including rice, wheat, maize, and barley. Availability of whole genome sequence information for number of crops along with the advancement in genome-editing techniques provides several possibilities to achieve desirable traits. In this review, the options available for crop improvement by implementing CRISPR/Cas9 based genome-editing techniques with special emphasis on cereal crops have been summarized. Recent advances providing opportunities to simultaneously edit many target genes were also discussed. The review also addressed recent advancements enabling precise base editing and gene expression modifications. In addition, the article also highlighted limitations such as transformation efficiency, specific promoters and most importantly the ethical and regulatory issues related to commercial release of novel crop varieties developed through genome editing. View Full-Text
Keywords: Biotic/abiotic stress; cereals; CRISPR/Cas9; genome editing; non-GMO Biotic/abiotic stress; cereals; CRISPR/Cas9; genome editing; non-GMO
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MDPI and ACS Style

Ansari, W.A.; Chandanshive, S.U.; Bhatt, V.; Nadaf, A.B.; Vats, S.; Katara, J.L.; Sonah, H.; Deshmukh, R. Genome Editing in Cereals: Approaches, Applications and Challenges. Int. J. Mol. Sci. 2020, 21, 4040. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114040

AMA Style

Ansari WA, Chandanshive SU, Bhatt V, Nadaf AB, Vats S, Katara JL, Sonah H, Deshmukh R. Genome Editing in Cereals: Approaches, Applications and Challenges. International Journal of Molecular Sciences. 2020; 21(11):4040. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114040

Chicago/Turabian Style

Ansari, Waquar A., Sonali U. Chandanshive, Vacha Bhatt, Altafhusain B. Nadaf, Sanskriti Vats, Jawahar L. Katara, Humira Sonah, and Rupesh Deshmukh. 2020. "Genome Editing in Cereals: Approaches, Applications and Challenges" International Journal of Molecular Sciences 21, no. 11: 4040. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21114040

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