The Influence of Bisphenol a on the Nitrergic Nervous Structures in the Domestic Porcine Uterus
Round 1
Reviewer 1 Report
The article "The influence od bisphenol a on the nitrergic nervous structures in the Uterus" is quite well conceived, however several modifications should be made to improve it:
-The order of subsection is not campatibel with the requirment of IJMS
-why the authors used these and not other doses of BPA ? it should be clarified in "Material and Methods"
-Table 1must be suplemmented with additional information, including town and country of producer, dilutions of antibodies and animal species of secondary antiodies
-In "Materials and methods" the authors wrote that animals were divided into control (C), experimental I and II groups (Ex I, Ex II), but in tables 1 and 2 results have been obtained from E1 and E2, plaese correct it
-The authors wrote that microscope was equipped with "appopriate IF filter sets". The exact type of filters should be mentioned.
-Tables 2 and 3 are not readable. The letters and digits are to small. Tables should be reedited.
-A scale bar should be added into each photograph
-A complete, extensively Revision of the English lanuage should be performed
Author Response
The authors thank reviewers for insightful reviews, which have contributed to improving the quality of the manuscript. All suggestions of the Reviewers were taken into account.
-why the authors used these and not other doses of BPA ? it should be clarified in "Material and Methods"
The text has been supplemented by: „The lower dose of BPA used in the present study is a dose which, in many countries, is recognized as a tolerable daily intake (TDI) or reference dose for BPA and is therefore considered to be safe for humans and animals [63]. In turn, the higher dose of BPA used in the present study is a dose where changes in the neurochemical characterization are clearly visible in the nervous structures supplying the gastrointestinal tract [39]”.
- Table 1must be suplemmented with additional information, including town and country of producer, dilutions of antibodies and animal species of secondary antiodies
-Tables 2 and 3 are not readable. The letters and digits are to small. Tables should be reedited.
The table has been reedited.
-In "Materials and methods" the authors wrote that animals were divided into control (C), experimental I and II groups (Ex I, Ex II), but in tables 1 and 2 results have been obtained from E1 and E2, plaese correct it
The materials and methods has been corrected.
-The authors wrote that microscope was equipped with "appopriate IF filter sets". The exact type of filters should be mentioned.
-A scale bar should be added into each photograph
The authors, according to the Reviewer’s suggestion, added scale bar and more information about filters.
-A complete, extensively Revision of the English lanuage should be performer
The text of the manuscript has been edited as regards language style by native speaker – worker of specialized translational office, specialist in the field of biological sciences.
The authors hope that corrections of the text and explanations will allow to publish the manuscript in “International Journal of Molecular Science”.
Reviewer 2 Report
Title: The influence of bisphenol a on the nitrergic nervous structures in the uterus
Authors : Rytel L and Gonkowski S.
Overview:
The aim of this study was to examine the influence of various doses of BPA of nitrergic nerves supplying the uterus with double immunofluorescence method.
The present study documented the specific NO, SP, VIP, PACAP and CART expression patterns under the influence of BPA (under the impact low and a high dose of bisphenol)
This study shows, for the first time, that even low doses of BPA result in the changes both in the number of nitrergic nerves in the uterine wall, as well as the degree of co-localization of NO with other neuronal substances.
Authors suggest that observed changes are the answer to the neurotoxic and/or pro-inflammatory activity of BPA and are connected with protective processes.
In general, the methods used are appropriate, and appear to have been carefully carried out.
Specific comments:
- The title of the manuscript must be reedited. Authors studied distribution of nerves in the domestic pig and this fact must be highlighted in the title
- Lists of abbreviation used in the text added at the beginning of the manuscript would increase its readability
- Figure legends are not clear. They mentioned photographs a, b and c, while on photographs letters A, B and C are presented. Figure legenda are too long. I suggest to reduce them (if it is possible)
- Some values in tables 2 and 3 and in the text are given to two decimal places, and other only to one decimal place. Why?
- Size units of values presented in tables 2 and 3 must be added in legends.
- Relatively large number of references cited in the text are out of dates (they have been published 15-20 years ago). They should be replaced by new research results in recent year.
- There are many spelling and grammatical mistakes in the text. It is better to be checked by English native speaker
Author Response
The authors thank reviewers for insightful reviews, which have contributed to improving the quality of the manuscript. All suggestions of the Reviewers were taken into account.
-The title of the manuscript must be reedited. Authors studied distribution of nerves in the domestic pig and this fact must be highlighted in the title
The title has been changed accoring to the Reviewers’s suggestion.
Lists of abbreviation used in the text added at the beginning of the manuscript would increase its readability
The list of abberevation has been added
- Figure legends are not clear. They mentioned photographs a, b and c, while on photographs letters A, B and C are presented. Figure legenda are too long. I suggest to reduce them (if it is possible)
- Some values in tables 2 and 3 and in the text are given to two decimal places, and other only to one decimal place. Why?
- Size units of values presented in tables 2 and 3 must be added in legends.
This part of the manuscript has been thoroughly reedited
Relatively large number of references cited in the text are out of dates (they have been published 15-20 years ago). They should be replaced by new research results in recent year.
The oldest citation has been repleced by researarch pulished in last years
There are many spelling and grammatical mistakes in the text. It is better to be checked by English native speaker
The text of the manuscript has been edited as regards language style by native speaker – worker of specialized translational office, specialist in the field of biological sciences.
The authors hope that corrections of the text and explanations will allow to publish the manuscript in “International Journal of Molecular Science”.
