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Article

Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies

1
Department of Dental Materials and Prosthodontics, Institute of Science and Technology, Sao Paulo State University, UNESP, Sao Paulo 12.245-700, Brazil
2
Department of Bioscience and Buccal Diagnose, Institute of Science and Technology, Sao Paulo State University, UNESP, Sao Paulo 12.245-700, Brazil
3
Department of Chemistry, University of Coimbra, CQC, 3004-535 Coimbra, Portugal
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(12), 4561; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21124561
Received: 15 June 2020 / Accepted: 24 June 2020 / Published: 26 June 2020
Chitosan-DNA (CS-DNA) and Chitosan-Pectin (CS-P) hydrogels were formulated as a sustained drug delivery carrier for drug delivery. For this, hydrogels were prepared by emulsion technique: mixing aqueous phase of the CS and DNA or P solution with benzyl alcohol using a high-performance dispersing instrument. Green Propolis (GP) was incorporated by imbibition: hydrogels were placed in GP aqueous solution (70 µg/mL) for 2 h. The specimens were freeze-dried and then characterized using different techniques. In vitro cell viability and morphology were also performed using the MG63 cell line. The presence of P was evidenced by the occurrence of a strong band at 1745 cm−1, also occurring in the blend. DNA and CS-DNA showed a strong band at 1650 cm−1, slightly shifted from the chitosan band. The sorption of GP induced a significant modification of the gel surface morphology and some phase separation occurs between chitosan and DNA. Drug release kinetics in water and in saliva follow a two-step mechanism. Significant biocompatibility revealed that these hydrogels were non-toxic and provided acceptable support for cell survival. Thus, the hydrogel complexation of chitosan with DNA and with Pectin provides favorable micro-environment for cell growth and is a viable alternative drug delivery system for Green Propolis. View Full-Text
Keywords: hydrogel; chitosan; pectin; DNA; propolis hydrogel; chitosan; pectin; DNA; propolis
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MDPI and ACS Style

Sato, T.; Mello, D.; Vasconcellos, L.; Valente, A.J.M.; Borges, A. Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies. Int. J. Mol. Sci. 2020, 21, 4561. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21124561

AMA Style

Sato T, Mello D, Vasconcellos L, Valente AJM, Borges A. Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies. International Journal of Molecular Sciences. 2020; 21(12):4561. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21124561

Chicago/Turabian Style

Sato, Tabata, Daphne Mello, Luana Vasconcellos, Artur J.M. Valente, and Alexandre Borges. 2020. "Chitosan-Based Coacervate Polymers for Propolis Encapsulation: Release and Cytotoxicity Studies" International Journal of Molecular Sciences 21, no. 12: 4561. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21124561

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