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Reply published on 12 September 2020, see Int. J. Mol. Sci. 2020, 21(18), 6675.
Comment published on 12 September 2020, see Int. J. Mol. Sci. 2020, 21(18), 6674.
Article

Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration

1
Department of Medical Biotechnology and Translational Medicine, Università degli Studi di Milano, 20129 Milano, Italy
2
Department of Pharmaceutical Sciences, Università degli Studi di Milano, 20133 Milano, Italy
3
Department of Health, Animal Science and Food Safety, Università degli Studi di Milano, 20133 Milano, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(13), 4661; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21134661
Received: 31 January 2020 / Revised: 23 June 2020 / Accepted: 29 June 2020 / Published: 30 June 2020
(This article belongs to the Special Issue Role of Nutraceuticals in Metabolic and Gastrointestinal Disorders)
Bone remodeling and repair require osteogenic cells to reach the sites that need to be rebuilt, indicating that stimulation of osteoblast migration could be a promising osteoanabolic strategy. We showed that purified δ-tocotrienol (δ-TT, 10 μg/mL), isolated from commercial palm oil (Elaeis guineensis) fraction, stimulates the migration of both MC3T3-E1 osteoblast-like cells and primary human bone marrow mesenchymal stem cells (BMSC) as detected by wound healing assay or Boyden chamber assay respectively. The ability of δ-TT to promote MC3T3-E1 cells migration is dependent on Akt phosphorylation detected by Western blotting and involves Wnt/β-catenin signalling pathway activation. In fact, δ-TT increased β-catenin transcriptional activity, measured using a Nano luciferase assay and pretreatment with procaine (2 µM), an inhibitor of the Wnt/β-catenin signalling pathway, reducing the wound healing activity of δ-TT on MC3T3-E1 cells. Moreover, δ-TT treatment increased the expression of β-catenin specific target genes, such as Osteocalcin and Bone Morphogenetic Protein-2, involved in osteoblast differentiation and migration, and increased alkaline phosphatase and collagen content, osteoblast differentiation markers. The ability of δ-TT to enhance the recruitment of BMSC, and to promote MC3T3-E1 differentiation and migratory behavior, indicates that δ-TT could be considered a promising natural anabolic compound. View Full-Text
Keywords: delta-tocotrienol; MC3T3-E1 cells; osteoblast migration; Wnt/β-catenin pathway; H3 and H4 acetylation level delta-tocotrienol; MC3T3-E1 cells; osteoblast migration; Wnt/β-catenin pathway; H3 and H4 acetylation level
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MDPI and ACS Style

Casati, L.; Pagani, F.; Maggi, R.; Ferrucci, F.; Sibilia, V. Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration. Int. J. Mol. Sci. 2020, 21, 4661. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21134661

AMA Style

Casati L, Pagani F, Maggi R, Ferrucci F, Sibilia V. Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration. International Journal of Molecular Sciences. 2020; 21(13):4661. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21134661

Chicago/Turabian Style

Casati, Lavinia, Francesca Pagani, Roberto Maggi, Francesco Ferrucci, and Valeria Sibilia. 2020. "Food for Bone: Evidence for a Role for Delta-Tocotrienol in the Physiological Control of Osteoblast Migration" International Journal of Molecular Sciences 21, no. 13: 4661. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21134661

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