Next Article in Journal
Modulating the Crosstalk between the Tumor and Its Microenvironment Using RNA Interference: A Treatment Strategy for Hepatocellular Carcinoma
Next Article in Special Issue
Clonal Expansion of Tumor-Infiltrating T Cells and Analysis of the Tumor Microenvironment within Esophageal Squamous Cell Carcinoma Relapsed after Definitive Chemoradiation Therapy
Previous Article in Journal
Phylogenomic proof of Recurrent Demipolyploidization and Evolutionary Stalling of the “Triploid Bridge” in Arundo (Poaceae)
Previous Article in Special Issue
Investigating Programmed Cell Death and Tumor Invasion in a Three-Dimensional (3D) Microfluidic Model of Glioblastoma
Article

Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections

1
UMR BIPAR, Ecole Nationale Vétérinaire d’Alfort, Laboratoire de santé animale d’Alfort, Anses, INRAE, Université Paris-Est, 94700 Maisons-Alfort, France
2
Center for Molecular Biophysics UPR4301 CNRS, 45000 Orléans, France
3
UMR Virologie, INRAE, Ecole Nationale Vétérinaire d’Alfort, Laboratoire de santé animale d’Alfort, Anses, Université Paris-Est, 94700 Maisons-Alfort, France
4
Laboratory of Molecular Oncology and Innovative Therapies, MIM, 04-141 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(15), 5249; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155249
Received: 24 June 2020 / Revised: 22 July 2020 / Accepted: 22 July 2020 / Published: 24 July 2020
(This article belongs to the Special Issue Recent Advances in Biotechnology)
Microvascular endothelial cells constitute potential targets for exogenous microorganisms, in particular for vector-borne pathogens. Their phenotypic and functional variations according to the organs they are coming from provide an explanation of the organ selectivity expressed in vivo by pathogens. In order to make available relevant tools for in vitro studies of infection mechanisms, our aim was to immortalize bovine organospecific endothelial cells but also to assess their permissivity to viral infection. Using transfection with SV40 large T antigen, six bovine microvascular endothelial cell lines from various organs and one macrovascular cell line from an umbilical cord were established. They display their own panel of endothelial progenitor/mature markers, as assessed by flow cytometry and RT-qPCR, as well as the typical angiogenesis capacity. Using both Bluetongue and foot-and-mouth disease viruses, we demonstrate that some cell lines are preferentially infected. In addition, they can be transfected and are able to express viral proteins such as BTV8-NS3. Such microvascular endothelial cell lines bring innovative tools for in vitro studies of infection by viruses or bacteria, allowing for the study of host-pathogen interaction mechanisms with the actual in vivo target cells. They are also suitable for applications linked to microvascularization, such as anti-angiogenic and anti-tumor research, growing fields in veterinary medicine. View Full-Text
Keywords: endothelium organospecificity; microvasculature; immortalization; cattle diseases; host-pathogen interactions; viruses endothelium organospecificity; microvasculature; immortalization; cattle diseases; host-pathogen interactions; viruses
Show Figures

Figure 1

MDPI and ACS Style

Lagrée, A.-C.; Fasani, F.; Rouxel, C.; Pivet, M.; Pourcelot, M.; Fablet, A.; Romey, A.; Caignard, G.; Vitour, D.; Blaise-Boisseau, S.; Kieda, C.; Boulouis, H.-J.; Haddad, N.; Grillon, C. Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections. Int. J. Mol. Sci. 2020, 21, 5249. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155249

AMA Style

Lagrée A-C, Fasani F, Rouxel C, Pivet M, Pourcelot M, Fablet A, Romey A, Caignard G, Vitour D, Blaise-Boisseau S, Kieda C, Boulouis H-J, Haddad N, Grillon C. Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections. International Journal of Molecular Sciences. 2020; 21(15):5249. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155249

Chicago/Turabian Style

Lagrée, Anne-Claire, Fabienne Fasani, Clotilde Rouxel, Marine Pivet, Marie Pourcelot, Aurore Fablet, Aurore Romey, Grégory Caignard, Damien Vitour, Sandra Blaise-Boisseau, Claudine Kieda, Henri-Jean Boulouis, Nadia Haddad, and Catherine Grillon. 2020. "Bovine Organospecific Microvascular Endothelial Cell Lines as New and Relevant In Vitro Models to Study Viral Infections" International Journal of Molecular Sciences 21, no. 15: 5249. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21155249

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop