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Advances in Molecular Dynamics Simulations and Enhanced Sampling Methods for the Study of Protein Systems
Article

Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s

Department of Cell and Molecular Biology, Uppsala University, Box-596, Biomedical Center, 751 24 Uppsala, Sweden
*
Author to whom correspondence should be addressed.
Present address: Department of Molecular Sciences, Uppsala Biocenter, Swedish University of Agricultural Sciences, 750 07 Uppsala, Sweden.
Int. J. Mol. Sci. 2020, 21(17), 6340; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176340
Received: 25 August 2020 / Accepted: 28 August 2020 / Published: 1 September 2020
(This article belongs to the Special Issue Advanced Research in Ribosomal RNAs)
The role of the nucleic acids in prion aggregation/disaggregation is becoming more and more evident. Here, using HET-s prion from fungi Podospora anserina (P. anserina) as a model system, we studied the role of RNA, particularly of different domains of the ribosomal RNA (rRNA), in its aggregation process. Our results using Rayleigh light scattering, Thioflavin T (ThT) binding, transmission electron microscopy (TEM) and cross-seeding assay show that rRNA, in particular the domain V of the major rRNA from the large subunit of the ribosome, substantially prevents insoluble amyloid and amorphous aggregation of the HET-s prion in a concentration-dependent manner. Instead, it facilitates the formation of the soluble oligomeric “seeds”, which are capable of promoting de novo HET-s aggregation. The sites of interactions of the HET-s prion protein on domain V rRNA were identified by primer extension analysis followed by UV-crosslinking, which overlap with the sites previously identified for the protein-folding activity of the ribosome (PFAR). This study clarifies a missing link between the rRNA-based PFAR and the mode of propagation of the fungal prions. View Full-Text
Keywords: ribosomal RNA; prion aggregation; P. anserina prion protein HET-s; PFAR ribosomal RNA; prion aggregation; P. anserina prion protein HET-s; PFAR
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MDPI and ACS Style

Pang, Y.; Kovachev, P.; Sanyal, S. Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s. Int. J. Mol. Sci. 2020, 21, 6340. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176340

AMA Style

Pang Y, Kovachev P, Sanyal S. Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s. International Journal of Molecular Sciences. 2020; 21(17):6340. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176340

Chicago/Turabian Style

Pang, Yanhong, Petar Kovachev, and Suparna Sanyal. 2020. "Ribosomal RNA Modulates Aggregation of the Podospora Prion Protein HET-s" International Journal of Molecular Sciences 21, no. 17: 6340. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21176340

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