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Article

Conserved and Opposite Transcriptome Patterns during Germination in Hordeum vulgare and Arabidopsis thaliana

1
Forage Seed Lab, Key Laboratory of Pratacultural Science, China Agricultural University, Beijing 100193, China
2
Department of Animal, Plant and Soil Science, School of Life Science, La Trobe University, Bundoora, Victoria 3086, Australia
3
Australian Research Council Centre of Excellence in Plant Energy Biology, La Trobe University, Bundoora, Victoria 3086, Australia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7404; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197404
Received: 2 September 2020 / Revised: 27 September 2020 / Accepted: 28 September 2020 / Published: 7 October 2020
(This article belongs to the Special Issue Plant Respiration)
Seed germination is a critical process for completion of the plant life cycle and for global food production. Comparing the germination transcriptomes of barley (Hordeum vulgare) to Arabidopsis thaliana revealed the overall pattern was conserved in terms of functional gene ontology; however, many oppositely responsive orthologous genes were identified. Conserved processes included a set of approximately 6000 genes that peaked early in germination and were enriched in processes associated with RNA metabolism, e.g., pentatricopeptide repeat (PPR)-containing proteins. Comparison of orthologous genes revealed more than 3000 orthogroups containing almost 4000 genes that displayed similar expression patterns including functions associated with mitochondrial tricarboxylic acid (TCA) cycle, carbohydrate and RNA/DNA metabolism, autophagy, protein modifications, and organellar function. Biochemical and proteomic analyses indicated mitochondrial biogenesis occurred early in germination, but detailed analyses revealed the timing involved in mitochondrial biogenesis may vary between species. More than 1800 orthogroups representing 2000 genes displayed opposite patterns in transcript abundance, representing functions of energy (carbohydrate) metabolism, photosynthesis, protein synthesis and degradation, and gene regulation. Differences in expression of basic-leucine zippers (bZIPs) and Apetala 2 (AP2)/ethylene-responsive element binding proteins (EREBPs) point to differences in regulatory processes at a high level, which provide opportunities to modify processes in order to enhance grain quality, germination, and storage as needed for different uses. View Full-Text
Keywords: Arabidopsis thaliana; Hordeum vulgare; mitochondria; energy metabolism photosynthesis; seed germination; transcriptome Arabidopsis thaliana; Hordeum vulgare; mitochondria; energy metabolism photosynthesis; seed germination; transcriptome
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MDPI and ACS Style

Zhu, Y.; Berkowitz, O.; Selinski, J.; Hartmann, A.; Narsai, R.; Wang, Y.; Mao, P.; Whelan, J. Conserved and Opposite Transcriptome Patterns during Germination in Hordeum vulgare and Arabidopsis thaliana. Int. J. Mol. Sci. 2020, 21, 7404. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197404

AMA Style

Zhu Y, Berkowitz O, Selinski J, Hartmann A, Narsai R, Wang Y, Mao P, Whelan J. Conserved and Opposite Transcriptome Patterns during Germination in Hordeum vulgare and Arabidopsis thaliana. International Journal of Molecular Sciences. 2020; 21(19):7404. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197404

Chicago/Turabian Style

Zhu, Yanqiao, Oliver Berkowitz, Jennifer Selinski, Andreas Hartmann, Reena Narsai, Yan Wang, Peisheng Mao, and James Whelan. 2020. "Conserved and Opposite Transcriptome Patterns during Germination in Hordeum vulgare and Arabidopsis thaliana" International Journal of Molecular Sciences 21, no. 19: 7404. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21197404

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