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Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer

Biomedical Research Institute INCLIVA, 46010 Valencia, Spain
Cancer Biology and Epigenetics Group–Research Center, Portuguese Oncology Institute of Porto (CI-IPOP), 4200-072 Porto, Portugal
Clinical Oncology Department, Hospital Clínico Universitario de Valencia, 46010 Valencia, Spain
Centro de Investigación Biomédica en Red de Cáncer (CIBERONC), 28029 Madrid, Spain
Department of Medicine, Universitat de València, 46010 Valencia, Spain
Department of Pathology, Portuguese Oncology Institute of Porto, 4200-072 Porto, Portugal
Department of Pathology and Molecular Immunology, Institute of Biomedical Sciences Abel Salazar-University of Porto (ICBAS-UP), 4050-313 Porto, Portugal
COST Action CA15204, 1210 Brussels, Belgium
Department of Physiology, Universitat de València, 46010 Valencia, Spain
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(19), 7427;
Received: 17 September 2020 / Revised: 2 October 2020 / Accepted: 6 October 2020 / Published: 8 October 2020
(This article belongs to the Section Biochemistry)
MicroRNAs have emerged as new diagnostic and therapeutic biomarkers for breast cancer. Herein, we analysed miR-99a-5p expression levels in primary tumours and plasma of breast cancer patients to evaluate its usefulness as a minimally invasive diagnostic biomarker. MiR-99a-5p expression levels were determined by quantitative real-time PCR in three independent cohorts of patients: (I) Discovery cohort: breast cancer tissues (n = 103) and healthy breast tissues (n = 26); (II) Testing cohort: plasma samples from 105 patients and 98 healthy donors; (III) Validation cohort: plasma samples from 89 patients and 85 healthy donors. Our results demonstrated that miR-99a-5p was significantly downregulated in breast cancer tissues compared to healthy breast tissues. Conversely, miR-99a-5p levels were significantly higher in breast cancer patients than in healthy controls in plasma samples from both testing and validation cohorts, and ROC curve analysis revealed that miR-99a-5p has good diagnostic potential even to detect early breast cancer. In conclusion, miR-99a-5p’s deregulated expression distinguished healthy patients from breast cancer patients in two different types of samples (tissues and plasma). Interestingly, expression levels in plasma were significantly lower in healthy controls than in early-stage breast cancer patients. Our findings suggest circulating miR-99a-5p as a novel promising non-invasive biomarker for breast cancer detection. View Full-Text
Keywords: breast cancer; biomarker; plasma; diagnosis breast cancer; biomarker; plasma; diagnosis
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MDPI and ACS Style

Garrido-Cano, I.; Constâncio, V.; Adam-Artigues, A.; Lameirinhas, A.; Simón, S.; Ortega, B.; Martínez, M.T.; Hernando, C.; Bermejo, B.; Lluch, A.; Lopes, P.; Henrique, R.; Jerónimo, C.; Cejalvo, J.M.; Eroles, P. Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer. Int. J. Mol. Sci. 2020, 21, 7427.

AMA Style

Garrido-Cano I, Constâncio V, Adam-Artigues A, Lameirinhas A, Simón S, Ortega B, Martínez MT, Hernando C, Bermejo B, Lluch A, Lopes P, Henrique R, Jerónimo C, Cejalvo JM, Eroles P. Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer. International Journal of Molecular Sciences. 2020; 21(19):7427.

Chicago/Turabian Style

Garrido-Cano, Iris, Vera Constâncio, Anna Adam-Artigues, Ana Lameirinhas, Soraya Simón, Belen Ortega, María T. Martínez, Cristina Hernando, Begoña Bermejo, Ana Lluch, Paula Lopes, Rui Henrique, Carmen Jerónimo, Juan M. Cejalvo, and Pilar Eroles. 2020. "Circulating miR-99a-5p Expression in Plasma: A Potential Biomarker for Early Diagnosis of Breast Cancer" International Journal of Molecular Sciences 21, no. 19: 7427.

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