Next Article in Journal
SIRT3 Acts as a Positive Autophagy Regulator to Promote Lipid Mobilization in Adipocytes via Activating AMPK
Next Article in Special Issue
Identification and Characterization of a β-N-Acetylhexosaminidase with a Biosynthetic Activity from the Marine Bacterium Paraglaciecola hydrolytica S66T
Previous Article in Journal
Profiling of O-acetylated Gangliosides Expressed in Neuroectoderm Derived Cells
Previous Article in Special Issue
Characterization of a Maltase from an Early-Diverged Non-Conventional Yeast Blastobotrys adeninivorans
Article

Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose

1
State Key Laboratory of Agrobiotechnology and Key Laboratory of Soil Microbiology, Ministry of Agriculture, College of Biological Sciences, China Agricultural University, Beijing 100193, China
2
Fujian Institute of Microbiology, Fuzhou 350007, China
3
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11340, Mexico
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 371; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020371
Received: 9 November 2019 / Revised: 31 December 2019 / Accepted: 2 January 2020 / Published: 7 January 2020
Fermentable sugars are important intermediate products in the conversion of lignocellulosic biomass to biofuels and other value-added bio-products. The main bottlenecks limiting the production of fermentable sugars from lignocellulosic biomass are the high cost and the low saccharification efficiency of degradation enzymes. Herein, we report the secretome of Trichoderma harzianum EM0925 under induction of lignocellulose. Numerously and quantitatively balanced cellulases and hemicellulases, especially high levels of glycosidases, could be secreted by T. harzianum EM0925. Compared with the commercial enzyme preparations, the T. harzianum EM0925 enzyme cocktail presented significantly higher lignocellulolytic enzyme activities and hydrolysis efficiency against lignocellulosic biomass. Moreover, 100% yields of glucose and xylose were obtained simultaneously from ultrafine grinding and alkali pretreated corn stover. These findings demonstrate a natural cellulases and hemicellulases mixture for complete conversion of biomass polysaccharide, suggesting T. harzianum EM0925 enzymes have great potential for industrial applications. View Full-Text
Keywords: glycoside hydrolyase; Trichoderma harzianum; complete saccharification; lignocellulose glycoside hydrolyase; Trichoderma harzianum; complete saccharification; lignocellulose
Show Figures

Figure 1

MDPI and ACS Style

Zhang, Y.; Yang, J.; Luo, L.; Wang, E.; Wang, R.; Liu, L.; Liu, J.; Yuan, H. Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose. Int. J. Mol. Sci. 2020, 21, 371. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020371

AMA Style

Zhang Y, Yang J, Luo L, Wang E, Wang R, Liu L, Liu J, Yuan H. Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose. International Journal of Molecular Sciences. 2020; 21(2):371. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020371

Chicago/Turabian Style

Zhang, Yu, Jinshui Yang, Lijin Luo, Entao Wang, Ruonan Wang, Liang Liu, Jiawen Liu, and Hongli Yuan. 2020. "Low-Cost Cellulase-Hemicellulase Mixture Secreted by Trichoderma harzianum EM0925 with Complete Saccharification Efficacy of Lignocellulose" International Journal of Molecular Sciences 21, no. 2: 371. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020371

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop