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Article

Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes

1
Key Laboratory of Crop Heterosis and Utilization (MOE) and State Key Laboratory for Agrobiotechnology, Beijing Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100193, China
2
Institute of Evolution, University of Haifa, 199 Abba-Hushi Avenue, Mt. Carmel, Haifa 3498838, Israel
3
Ministry of Education Key Laboratory of Molecular and Cellular Biology, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(2), 673; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020673
Received: 8 December 2019 / Revised: 14 January 2020 / Accepted: 17 January 2020 / Published: 20 January 2020
(This article belongs to the Special Issue Abiotic Stress and Gene Networks in Plants 2020)
Our previous study indicated that glycerol application induced resistance to powdery mildew (Bgt) in wheat by regulating two important signal molecules, glycerol-3-phosphate (G3P) and oleic acid (OA18:1). Transcriptome analysis of wheat leaves treated by glycerol and inoculated with Bgt was performed to identify the activated immune response pathways. We identified a set of differentially expressed transcripts (e.g., TaGLI1, TaACT1, and TaSSI2) involved in glycerol and fatty acid metabolism that were upregulated in response to Bgt infection and might contribute to G3P and OA18:1 accumulation. Gene Ontology (GO) enrichment analysis revealed GO terms induced by glycerol, such as response to jasmonic acid (JA), defense response to bacterium, lipid oxidation, and growth. In addition, glycerol application induced genes (e.g., LOX, AOS, and OPRs) involved in the metabolism pathway of linolenic and alpha-linolenic acid, which are precursor molecules of JA biosynthesis. Glycerol induced JA and salicylic acid (SA) levels, while glycerol reduced the auxin (IAA) level in wheat. Glycerol treatment also induced pathogenesis related (PR) genes, including PR-1, PR-3, PR-10, callose synthase, PRMS, RPM1, peroxidase, HSP70, HSP90, etc. These results indicate that glycerol treatment regulates fatty acid metabolism and hormones cross-talk and induces the expression of PR genes that together contribute to Bgt resistance in wheat. View Full-Text
Keywords: disease resistance; glycerol application; Triticum aestivum L.; transcriptome sequencing disease resistance; glycerol application; Triticum aestivum L.; transcriptome sequencing
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MDPI and ACS Style

Li, Y.; Qiu, L.; Liu, X.; Zhang, Q.; Zhuansun, X.; Fahima, T.; Krugman, T.; Sun, Q.; Xie, C. Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes. Int. J. Mol. Sci. 2020, 21, 673. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020673

AMA Style

Li Y, Qiu L, Liu X, Zhang Q, Zhuansun X, Fahima T, Krugman T, Sun Q, Xie C. Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes. International Journal of Molecular Sciences. 2020; 21(2):673. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020673

Chicago/Turabian Style

Li, Yinghui, Lina Qiu, Xinye Liu, Qiang Zhang, Xiangxi Zhuansun, Tzion Fahima, Tamar Krugman, Qixin Sun, and Chaojie Xie. 2020. "Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes" International Journal of Molecular Sciences 21, no. 2: 673. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21020673

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