Genome-Wide Identification of the Gossypium hirsutum NHX Genes Reveals That the Endosomal-Type GhNHX4A Is Critical for the Salt Tolerance of Cotton
Abstract
:1. Introduction
2. Results
2.1. Genome-Wide Identification and Phylogenetic Relationships of NHX Genes
2.2. Gene Structures and Conserved Motifs of NHXs in G. hirsutum
2.3. Expression Patterns of GhNHX Genes under a Salt Treament
2.4. Functional Complementation of GhNHX4A in Yeast Mutants
2.5. Subcellular Localization of GhNHX4A
2.6. Virus-Induced Gene Silencing of GhNHX4A in G. hirsutum
3. Discussion
3.1. Cotton NHX Gene Family
3.2. Expression Profiles of GhNHX Genes under the Salt Treatment
3.3. GhNHX4A-Mediated Salt Tolerance
4. Materials and Methods
4.1. Identification of NHX Genes in Gossypium Species
4.2. Phylogenetic Relationship, Chromosomal Localization, Gene Structure, and Conserved Motif Analyses
4.3. Plant Materials and Treaments
4.4. RNA Isolation and Quantitative Real-Time PCR Analysis
4.5. Functional Analysis of GhNHXs Using Yeast Mutants
4.6. Transient Expression of Enhanced Green Fluorescent Protein (eGFP) Constructs in Tobacco Leaves
4.7. Virus-Induced Gene Silencing Analyses in Cotton
4.8. Determination of Intracellular K+ and Na+ Contents
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CPA1 | cation/proton antiporter-1 |
NHX | Na+/H+ antiporter |
qPCR | quantitative real-time PCR |
VIGS | virus-induced gene silencing |
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Ma, W.; Ren, Z.; Zhou, Y.; Zhao, J.; Zhang, F.; Feng, J.; Liu, W.; Ma, X. Genome-Wide Identification of the Gossypium hirsutum NHX Genes Reveals That the Endosomal-Type GhNHX4A Is Critical for the Salt Tolerance of Cotton. Int. J. Mol. Sci. 2020, 21, 7712. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21207712
Ma W, Ren Z, Zhou Y, Zhao J, Zhang F, Feng J, Liu W, Ma X. Genome-Wide Identification of the Gossypium hirsutum NHX Genes Reveals That the Endosomal-Type GhNHX4A Is Critical for the Salt Tolerance of Cotton. International Journal of Molecular Sciences. 2020; 21(20):7712. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21207712
Chicago/Turabian StyleMa, Wenyu, Zhongying Ren, Yang Zhou, Junjie Zhao, Fei Zhang, Junping Feng, Wei Liu, and Xiongfeng Ma. 2020. "Genome-Wide Identification of the Gossypium hirsutum NHX Genes Reveals That the Endosomal-Type GhNHX4A Is Critical for the Salt Tolerance of Cotton" International Journal of Molecular Sciences 21, no. 20: 7712. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21207712