High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling
Abstract
:1. Introduction
2. Results and Discussions
3. Materials and Methods
Estimation of the Rate Constant
4. Conclusions
Supplementary Materials
Funding
Conflicts of Interest
Abbreviations
BWE | body water enrichment |
HRMS | high-resolution mass spectrometry |
LC–MS | liquid chromatography and mass spectrometry |
m/z | mass-to-charge ration |
NEH | number of exchangeable hydrogens |
QToF | quadrupole time-of-flight |
R | resolution |
RA | relative abundance |
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Number of | Resolution (×1000) | ||||
---|---|---|---|---|---|
30 | 60 | 120 | 240 | 480 | |
quantified proteins | 91 | 97 | 92 | 86 | 58 |
proteins with 3 or more unique peptides | 32 | 29 | 23 | 21 | 13 |
proteins with CV ≤ 0.3 | 45 | 41 | 39 | 26 | 10 |
peptides usable for proteome dynamics | 454 | 472 | 421 | 363 | 219 |
identified but unquantifiable proteins | 13 | 10 | 16 | 17 | 35 |
Proteins | QToF1 | QToF2 | Resolution (×1000) | Linear Coefficient (×10−3) | ||||
---|---|---|---|---|---|---|---|---|
30 | 60 | 120 | 240 | 480 | ||||
Albumin | 0.170 | 0.224 | 0.158 | 0.154 | 0.134 | 0.101 | 0.072 | −0.2 ** |
Serotransferrin | 0.391 | 0.455 | 0.340 | 0.344 | 0.329 | 0.244 | 0.168 | −0.4 ** |
Alpha-2-macroglobulin | 0.309 | 0.352 | 0.258 | 0.260 | 0.235 | 0.136 | 0.080 | −0.4 ** |
Hemopexin | 0.387 | 0.504 | 0.573 | 0.505 | 0.360 | 0.375 | 0.339 | −0.4 |
Apolipoprotein A-I | 0.629 | 0.570 | 0.768 | 0.654 | 0.599 | 0.438 | 0.069 | −1.5 ** |
Complement C3 | 1.277 | 2.08 | 1.019 | 1.174 | 1.169 | 0.788 | 0.142 | −2.2 * |
Carboxylesterase 1C | 0.522 | 0.215 | 0.625 | 0.414 | 0.538 | 0.392 | 0.300 | −0.5 |
Murinoglobulin-1 | 0.321 | 0.374 | 0.343 | 0.316 | 0.247 | 0.161 | −0.031 | −0.7 ** |
Serine protease inhibitor A3K | 0.466 | 0.497 | 0.466 | 0.453 | 0.412 | 0.284 | 0.193 | −0.6 ** |
Transthyretin | 1.087 | 1.203 | 1.095 | 1.02 | 0.877 | 0.95 | 0.620 | −0.9 * |
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Sadygov, R.G. High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling. Int. J. Mol. Sci. 2020, 21, 7821. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217821
Sadygov RG. High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling. International Journal of Molecular Sciences. 2020; 21(21):7821. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217821
Chicago/Turabian StyleSadygov, Rovshan G. 2020. "High-Resolution Mass Spectrometry for In Vivo Proteome Dynamics using Heavy Water Metabolic Labeling" International Journal of Molecular Sciences 21, no. 21: 7821. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217821