Next Article in Journal
Transcriptomic Analysis of Age-Associated Periventricular Lesions Reveals Dysregulation of the Immune Response
Next Article in Special Issue
Skeletal Muscle Recovery from Disuse Atrophy: Protein Turnover Signaling and Strategies for Accelerating Muscle Regrowth
Previous Article in Journal
Heterogeneity of Neuroinflammatory Responses in Amyotrophic Lateral Sclerosis: A Challenge or an Opportunity?
Previous Article in Special Issue
Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia
Article

Sarcopenia Induced by Chronic Liver Disease in Mice Requires the Expression of the Bile Acids Membrane Receptor TGR5

1
Laboratory of Muscle Pathology, Fragility and Aging, Department of Biological Sciences, Faculty of Life Sciences, Universidad Andres Bello, Santiago 8370146, Chile
2
Millennium Institute on Immunology and Immunotherapy, Santiago 8370146, Chile
3
Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Universidad de Santiago de Chile, Santiago 8350709, Chile
4
Millennium Nucleus of Ion Channels-Associated Diseases (MiNICAD), Universidad de Chile, Santiago 8370146, Chile
5
Laboratory of Integrative Physiopathology, Department of Biological Sciences, Faculty of Life Sciences, Universidad Andres Bello, Santiago 8370146, Chile
6
Centro de Envejecimiento y Regeneración (CARE), Departamento de Gastroenterología, Escuela de Medicina, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, Santiago 8330077, Chile
7
Facultad de Ciencias Médicas, Universidad Bernardo O Higgins, Santiago 8370993, Chile
8
Faculty of Medicine and Faculty of Life Sciences, Institute of Biomedical Sciences, Universidad Andres Bello, Santiago 8370146, Chile
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(21), 7922; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217922
Received: 18 September 2020 / Revised: 21 October 2020 / Accepted: 22 October 2020 / Published: 25 October 2020
(This article belongs to the Special Issue Muscle Atrophy: Discovery of Mechanisms and Potential Therapies)
Sarcopenia is a condition of muscle dysfunction, commonly associated with chronic liver disease (CLD), characterized by a decline in muscle strength, the activation of the ubiquitin-proteasome system (UPS), and oxidative stress. We recently described a murine model of CLD-induced sarcopenia by intake of hepatotoxin 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), which presents an increase in plasma bile acids (BA). BA induced skeletal muscle atrophy through a mechanism dependent on the Takeda G protein-coupled receptor 5 (TGR5) receptor. In the present study, we evaluated the role of TGR5 signaling in the development of sarcopenia using a model of DDC-induced CLD in C57BL6 wild-type (WT) mice and mice deficient in TGR5 expression (TGR5−/− mice). The results indicate that the decline in muscle function and contractibility induced by the DDC diet is dependent on TGR5 expression. TGR5 dependence was also observed for the decrease in fiber diameter and sarcomeric proteins, as well as for the fast-to-slow shift in muscle fiber type. UPS overactivation, indicated by increased atrogin-1/MAFbx (atrogin-1) and muscle RING-finger protein-1 (MuRF-1) protein levels and oxidative stress, was abolished in tibialis anterior muscles from TGR5−/− mice. Our results collectively suggest that all sarcopenia features induced by the DDC-supplemented diet in mice are dependent on TGR5 receptor expression. View Full-Text
Keywords: bile acids; muscle atrophy; TGR5 receptor; oxidative stress bile acids; muscle atrophy; TGR5 receptor; oxidative stress
Show Figures

Graphical abstract

MDPI and ACS Style

Abrigo, J.; Campos, F.; Gonzalez, F.; Aguirre, F.; Gonzalez, A.; Huerta-Salgado, C.; Conejeros, S.; Simon, F.; Arrese, M.; Cabrera, D.; Elorza, A.A.; Cabello-Verrugio, C. Sarcopenia Induced by Chronic Liver Disease in Mice Requires the Expression of the Bile Acids Membrane Receptor TGR5. Int. J. Mol. Sci. 2020, 21, 7922. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217922

AMA Style

Abrigo J, Campos F, Gonzalez F, Aguirre F, Gonzalez A, Huerta-Salgado C, Conejeros S, Simon F, Arrese M, Cabrera D, Elorza AA, Cabello-Verrugio C. Sarcopenia Induced by Chronic Liver Disease in Mice Requires the Expression of the Bile Acids Membrane Receptor TGR5. International Journal of Molecular Sciences. 2020; 21(21):7922. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217922

Chicago/Turabian Style

Abrigo, Johanna, Fabián Campos, Francisco Gonzalez, Francisco Aguirre, Andrea Gonzalez, Camila Huerta-Salgado, Sabrina Conejeros, Felipe Simon, Marco Arrese, Daniel Cabrera, Alvaro A. Elorza, and Claudio Cabello-Verrugio. 2020. "Sarcopenia Induced by Chronic Liver Disease in Mice Requires the Expression of the Bile Acids Membrane Receptor TGR5" International Journal of Molecular Sciences 21, no. 21: 7922. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217922

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop