Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments
Abstract
:1. Introduction
2. Results
2.1. BMP-2, but Not FGF-2, Promotes the Repair of Mouse Calvarial Defect
2.2. FGF-2, but Not BMP-2, Promotes the Repair of Mouse Femoral Defect
2.3. Depletion of Bone Marrow Cells Inhibits FGF-2-Induced Bone Formation in the Marrow Cavity
2.4. Effects of BMP-2 and FGF-2 on Osteoblast and Osteoclast in Mouse Calvarial and Femoral Defects
2.5. Effects of BMP-2 and FGF-2 on Angiogenesis in Mouse Calvarial and Femoral Defects
2.6. Effects of BMP-2 and FGF-2 on Bone Marrow Cell Populations
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Animal Experiments
4.3. Micro-CT Analysis
4.4. Histological Analysis
4.5. Flow Cytometry
5. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Antibody | Conjugation | Clone | Source | Cat.# |
---|---|---|---|---|
CD3e | APC | 145-2C11 | BioLegend | 100312 |
CD11b | APC | M1/70 | BioLegend | 101212 |
CD16/32 | 2.4G2 | BD Biosciences | 553142 | |
CD31 | APC | MEC13.3 | BioLegend | 102509 |
CD31 | PE | MEC13.3 | BioLegend | 102507 |
CD45 | APC | 30-F11 | BioLegend | 103111 |
CD45R (B220) | APC | RA3-6B2 | BioLegend | 103211 |
CD48 | BV421 | HM48-1 | BioLegend | 103428 |
CD117 (c-Kit) | FITC | 2B8 | BioLegend | 105806 |
CD150 | PE | TC15-12F12.2 | BioLegend | 115903 |
Leptin Receptor | Biotin | R&D systems | BAF497 | |
Lineage marker cocktail | APC | 145-2C11(CD3e), M1/70(CD11b), RA3-6B2 (CD45R/B220), TER-119 (Ly76), RB6-8C5 (Ly6G/C) | BD Biosciences | 51-9003632 |
Ly-6A/E (Sca-1) | PE-Cy7 | D7 | BioLegend | 108114 |
Ly-6G/C (Gr-1) | BV421 | RB6-8C5 | BD Biosciences | 562709 |
Streptavidin | BV421 | BioLegend | 405226 | |
Ter119 | APC | TER-119 | BioLegend | 116211 |
7-AAD | BioLegend | 420404 |
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Nosho, S.; Tosa, I.; Ono, M.; Hara, E.S.; Ishibashi, K.; Mikai, A.; Tanaka, Y.; Kimura-Ono, A.; Komori, T.; Maekawa, K.; et al. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments. Int. J. Mol. Sci. 2020, 21, 7967. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217967
Nosho S, Tosa I, Ono M, Hara ES, Ishibashi K, Mikai A, Tanaka Y, Kimura-Ono A, Komori T, Maekawa K, et al. Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments. International Journal of Molecular Sciences. 2020; 21(21):7967. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217967
Chicago/Turabian StyleNosho, Shuji, Ikue Tosa, Mitsuaki Ono, Emilio Satoshi Hara, Kei Ishibashi, Akihiro Mikai, Yukie Tanaka, Aya Kimura-Ono, Taishi Komori, Kenji Maekawa, and et al. 2020. "Distinct Osteogenic Potentials of BMP-2 and FGF-2 in Extramedullary and Medullary Microenvironments" International Journal of Molecular Sciences 21, no. 21: 7967. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21217967