Dietary Salt Administration Decreases Enterotoxigenic Bacteroides fragilis (ETBF)-Promoted Tumorigenesis via Inhibition of Colonic Inflammation
Abstract
:1. Introduction
2. Results
2.1. Effects of a High Salt Diet on Body Weight and Survival in ETBF-Colonized Mice
2.2. High Salt Diet Prevented Cecum Weight Loss and Spleen Enlargement in ETBF-Colonized Mice
2.3. High Salt Diet Reduced IL-17A and Nitric Oxide in ETBF-Colonized Mice
2.4. High Salt Diet Decreased Large Intestinal Inflammation in ETBF-Colonized Mice
2.5. High Salt Diet Reduced ETBF-Mediated Tumorigenesis in Azoxymethane (AOM)/Dextran Sulfate Sodium (DSS) Mice
2.6. Sodium Chloride Does Not Affect BFT-Induced Cell Rounding and IL-8 Expression in HT29/C1 Cells
3. Discussion
4. Materials and Methods
4.1. Mice
4.2. Cell Culture
4.3. Quantitative PCR
4.4. Nitric Oxide Assay
4.5. Preparation of Bacteria
4.6. Normal Salt Diet and High Salt Diet
4.7. Tumorigenesis Experiment
4.8. Histology
4.9. Cytokine Analysis
4.10. Bacteria Enumeration
4.11. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Histological Parameters | Description |
---|---|
Ulceration | A local defect, or excavation of the surface of an organ or tissue, produced by the sloughing of necrotic inflammatory tissue |
Crypt abscess | Aggregates of neutrophils, fibrin, and sloughed epithelial cells within a partially ruptured colonic gland |
Erosion | The superficial destruction of a surface by stimuli such as inflammation |
Hyperplasia | Abnormal increase in the volume of a tissue or organ caused by the formation and growth of new normal cells |
Infiltration | The pathological accumulation of immune cells in tissue |
Scoring system | 0, no lesion; 1, mild; 2, moderate; 3, strong |
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Hwang, S.; Yi, H.C.; Hwang, S.; Jo, M.; Rhee, K.-J. Dietary Salt Administration Decreases Enterotoxigenic Bacteroides fragilis (ETBF)-Promoted Tumorigenesis via Inhibition of Colonic Inflammation. Int. J. Mol. Sci. 2020, 21, 8034. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218034
Hwang S, Yi HC, Hwang S, Jo M, Rhee K-J. Dietary Salt Administration Decreases Enterotoxigenic Bacteroides fragilis (ETBF)-Promoted Tumorigenesis via Inhibition of Colonic Inflammation. International Journal of Molecular Sciences. 2020; 21(21):8034. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218034
Chicago/Turabian StyleHwang, Soonjae, Hye Chin Yi, Samnoh Hwang, Minjeong Jo, and Ki-Jong Rhee. 2020. "Dietary Salt Administration Decreases Enterotoxigenic Bacteroides fragilis (ETBF)-Promoted Tumorigenesis via Inhibition of Colonic Inflammation" International Journal of Molecular Sciences 21, no. 21: 8034. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218034