Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion
Abstract
:1. Introduction
2. Results and Discussion
2.1. Physiological Interplay between CAFs and Cancer Cells Promotes Cancer Cell Invasion
2.2. Genetic Analysis of HUVEC Differentiation into CAF by Exosomes
2.3. Genetic Analysis of HUVEC Differentiation into CAF by Cancer Cells
2.4. Genetic Analysis of HUVEC Differentiation into CAF by Both Exosomes and Cancer Cells
3. Materials and Methods
3.1. Cultivation of Cancer Cells
3.2. Isolation of Cancer Cell-Derived Exosomes
3.3. Characterizations of Exosomes
3.4. Preparation of 3D Microfluidic Cancer Microenvironment
3.5. Culturing of HUVECs in Microfluidic Devices
3.6. Cultivation of Cancer-Associated Fibroblasts (CAFs) in the Microfluidic Device
3.7. Cultivation of Invasive Cancer Cells in Microfluidic Device
3.8. Immunostaining of Cells
3.9. Quantification of Invasive Cell Number, Invaded Area and Penetrated Distance of CAFs and Cancer Cells
3.10. RNA Extraction and NanoString nCounter Assay
3.11. Module Analysis of the Protein–Protein Interaction (PPI) Network
3.12. GeneOntology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) Pathway Analyses
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Kim, K.; Sohn, Y.J.; Lee, R.; Yoo, H.J.; Kang, J.Y.; Choi, N.; Na, D.; Yeon, J.H. Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion. Int. J. Mol. Sci. 2020, 21, 8153. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218153
Kim K, Sohn YJ, Lee R, Yoo HJ, Kang JY, Choi N, Na D, Yeon JH. Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion. International Journal of Molecular Sciences. 2020; 21(21):8153. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218153
Chicago/Turabian StyleKim, Kimin, Yeh Joo Sohn, Ruri Lee, Hye Ju Yoo, Ji Yoon Kang, Nakwon Choi, Dokyun Na, and Ju Hun Yeon. 2020. "Cancer-Associated Fibroblasts Differentiated by Exosomes Isolated from Cancer Cells Promote Cancer Cell Invasion" International Journal of Molecular Sciences 21, no. 21: 8153. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21218153