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Article

In Vitro Interaction of 5-Aminoorotic Acid and Its Gallium(III) Complex with Superoxide Radical, Generated by Two Model Systems

1
Department of Chemistry, Faculty of Pharmacy, Medical University—Sofia, 1000 Sofia, Bulgaria
2
Department of Physics and Biophysics, Faculty of Medicine, Medical University—Sofia, 1431 Sofia, Bulgaria
3
Department of Physiology and Pharmacology “Vittorio Erspamer”, Faculty of Pharmacy and Medicine, Sapienza University, 00185 Rome, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(22), 8862; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21228862
Received: 8 October 2020 / Revised: 3 November 2020 / Accepted: 20 November 2020 / Published: 23 November 2020
Increased levels of the superoxide radical are associated with oxidative damage to healthy tissues and with elimination of malignant cells in a living body. It is desirable that a chemotherapeutic combines pro-oxidant behavior around and inside tumors with antioxidant action near healthy cells. A complex consisting of a pro-oxidant cation and antioxidant ligands could be a potential anticancer agent. Ga(III) salts are known anticancer substances, and 5-aminoorotic acid (HAOA) is a ligand with antioxidant properties. The in vitro effects of HAOA and its complex with Ga(III) (gallium(III) 5-aminoorotate (GaAOA)) on the in vitro accumulation of superoxide and other free radicals were estimated. Model systems such as potassium superoxide (KO2), xanthine/xanthine oxidase (X/XO), and rat blood serum were utilized. Data suggested better antioxidant effect of GaAOA compared to HAOA. Evidently, all three ligands of GaAOA participated in the scavenging of superoxide. The effects in rat blood serum were more nuanced, considering the chemical and biochemical complexity of this model system. It was observed that the free-radical-scavenging action of both compounds investigated may be manifested via both hydrogen donation and electron transfer pathways. It was proposed that the radical-scavenging activities (RSAs) of HAOA and its complex with Ga(III) may be due to a complex process, depending on the concentration, and on the environment, nature, and size of the free radical. The electron transfer pathway was considered as more probable in comparison to hydrogen donation in the scavenging of superoxide by 5-aminoorotic acid and its gallium(III) complex. View Full-Text
Keywords: oxidative stress; gallium complex; 5-aminoorotic acid; antioxidant; superoxide radical; potassium superoxide; xanthine/xanthine oxidase oxidative stress; gallium complex; 5-aminoorotic acid; antioxidant; superoxide radical; potassium superoxide; xanthine/xanthine oxidase
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MDPI and ACS Style

Todorov, L.; Traykova, M.; Saso, L.; Kostova, I. In Vitro Interaction of 5-Aminoorotic Acid and Its Gallium(III) Complex with Superoxide Radical, Generated by Two Model Systems. Int. J. Mol. Sci. 2020, 21, 8862. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21228862

AMA Style

Todorov L, Traykova M, Saso L, Kostova I. In Vitro Interaction of 5-Aminoorotic Acid and Its Gallium(III) Complex with Superoxide Radical, Generated by Two Model Systems. International Journal of Molecular Sciences. 2020; 21(22):8862. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21228862

Chicago/Turabian Style

Todorov, Lozan, Maria Traykova, Luciano Saso, and Irena Kostova. 2020. "In Vitro Interaction of 5-Aminoorotic Acid and Its Gallium(III) Complex with Superoxide Radical, Generated by Two Model Systems" International Journal of Molecular Sciences 21, no. 22: 8862. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21228862

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