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The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans-Prenyltransferase Structural Analysis, Expression and Localization

Department of Genetic Engineering and Graduate School of Biotechnology, College of Life Sciences, Kyung Hee University, Yongin 17104, Korea
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Int. J. Mol. Sci. 2020, 21(23), 8927; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21238927
Received: 28 September 2020 / Revised: 15 November 2020 / Accepted: 18 November 2020 / Published: 25 November 2020
(This article belongs to the Special Issue Biological Networks of Specialized Metabolites and Plants)
Most terpenoids are derived from the basic terpene skeletons of geranyl pyrophosphate (GPP, C10), farnesyl-PP (FPP, C15) and geranylgeranyl-PP (GGPP, C20). The trans-prenyltransferases (PTs) mediate the sequential head-to-tail condensation of an isopentenyl-PP (C5) with allylic substrates. The in silico structural comparative analyses of rice trans-PTs with 136 plant trans-PT genes allowed twelve rice PTs to be identified as GGPS_LSU (OsGGPS1), homomeric G(G)PS (OsGPS) and GGPS_SSU-II (OsGRP) in Group I; two solanesyl-PP synthase (OsSPS2 and 3) and two polyprenyl-PP synthases (OsSPS1 and 4) in Group II; and five FPSs (OsFPS1, 2, 3, 4 and 5) in Group III. Additionally, several residues in “three floors” for the chain length and several essential domains for enzymatic activities specifically varied in rice, potentiating evolutionarily rice-specific biochemical functions of twelve trans-PTs. Moreover, expression profiling and localization patterns revealed their functional compartmentation in rice. Taken together, we propose the predicted topology-based working model of rice PTs with corresponding terpene metabolites: GPP/GGPPs mainly in plastoglobuli, SPPs in stroma, PPPs in cytosol, mitochondria and chloroplast and FPPs in cytosol. Our findings could be suitably applied to metabolic engineering for producing functional terpene metabolites in rice systems. View Full-Text
Keywords: functional compartmentation; in silico structural analysis; rice (Oryza sativa); subcellular localization; topology-based working model; trans-prenyltransferase functional compartmentation; in silico structural analysis; rice (Oryza sativa); subcellular localization; topology-based working model; trans-prenyltransferase
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MDPI and ACS Style

You, M.K.; Lee, Y.J.; Yu, J.S.; Ha, S.-H. The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans-Prenyltransferase Structural Analysis, Expression and Localization. Int. J. Mol. Sci. 2020, 21, 8927. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21238927

AMA Style

You MK, Lee YJ, Yu JS, Ha S-H. The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans-Prenyltransferase Structural Analysis, Expression and Localization. International Journal of Molecular Sciences. 2020; 21(23):8927. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21238927

Chicago/Turabian Style

You, Min K., Yeo J. Lee, Ji S. Yu, and Sun-Hwa Ha. 2020. "The Predicted Functional Compartmentation of Rice Terpenoid Metabolism by Trans-Prenyltransferase Structural Analysis, Expression and Localization" International Journal of Molecular Sciences 21, no. 23: 8927. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21238927

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