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Article

Characterization and Transcriptome Analysis of Exosomal and Nonexosomal RNAs in Bovine Adipocytes

Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, Shaanxi, China
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Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(23), 9313; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21239313
Received: 13 November 2020 / Revised: 30 November 2020 / Accepted: 1 December 2020 / Published: 7 December 2020
(This article belongs to the Special Issue Functions of Non-coding DNA Regions)
Exosomes are endosome-derived extracellular vesicles that allow intercellular communication. However, the biological significance of adipocyte exosomal RNAs remains unclear. To determine the role of RNAs from bovine adipocytes and exosomes in bovine adipogenesis, exosomal and nonexosomal RNAs were extracted from three bovine primary white adipocyte samples and then profiles were generated using DNBSEQ/BGISEQ-500 technology. The RNAome of adipocytes consisted of 12,082 mRNAs, 8589 lncRNAs, and 378 miRNAs for a higher complexity that that detected in exosomes, with 1083 mRNAs, 105 lncRNAs, and 48 miRNAs. Exosomal miRNA-mRNA and lncRNA–miRNA–mRNA networks were constructed and enrichment analysis was performed to predict functional roles and regulatory mechanisms. Our study provides the first characterization of RNAs from bovine adipocyte and exosomes. The findings reveal that some RNAs are specifically packaged in adipocyte-derived exosomes, potentially enabling crosstalk between adipocytes and/or other cells that is mediated by exosomes. Our results greatly expand our understanding of exosomal RNAs from bovine adipocytes, and provide a reference for future functional investigations of adipocyte exosomal RNAs under normal physiological conditions. View Full-Text
Keywords: exosomal RNAs; adipocyte; bovine; RNA-seq exosomal RNAs; adipocyte; bovine; RNA-seq
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MDPI and ACS Style

Yue, B.; Yang, H.; Wu, J.; Wang, J.; Ru, W.; Cheng, J.; Huang, Y.; Lei, C.; Lan, X.; Chen, H. Characterization and Transcriptome Analysis of Exosomal and Nonexosomal RNAs in Bovine Adipocytes. Int. J. Mol. Sci. 2020, 21, 9313. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21239313

AMA Style

Yue B, Yang H, Wu J, Wang J, Ru W, Cheng J, Huang Y, Lei C, Lan X, Chen H. Characterization and Transcriptome Analysis of Exosomal and Nonexosomal RNAs in Bovine Adipocytes. International Journal of Molecular Sciences. 2020; 21(23):9313. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21239313

Chicago/Turabian Style

Yue, Binglin, Haiyan Yang, Jiyao Wu, Jian Wang, Wenxiu Ru, Jie Cheng, Yongzheng Huang, Chuzhao Lei, Xianyong Lan, and Hong Chen. 2020. "Characterization and Transcriptome Analysis of Exosomal and Nonexosomal RNAs in Bovine Adipocytes" International Journal of Molecular Sciences 21, no. 23: 9313. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21239313

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