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Article

Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation

1
Hematology Department-BMT Unit, G Papanicolaou Hospital, 57010 Thessaloniki, Greece
2
3rd Department of Internal Medicine, Papageorgiou Hospital, Aristotle University of Thessaloniki, 56403 Thessaloniki, Greece
3
Hematology Laboratory, Theagenion Cancer Center, 54007 Thessaloniki, Greece
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(24), 9768; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21249768
Received: 15 November 2020 / Revised: 18 December 2020 / Accepted: 18 December 2020 / Published: 21 December 2020
(This article belongs to the Special Issue Molecular Advances in Hypertension and Blood)
(1) Background: survivors of allogeneic hematopoietic cell transplantation (alloHCT) suffer from morbidity and mortality due to cardiovascular events. We hypothesized that vascular injury and pro-coagulant activity are evident in alloHCT survivors without existing alloHCT complications or relapse. (2) Methods: we enrolled consecutive adult alloHCT survivors without established cardiovascular disease and control individuals matched for traditional cardiovascular risk factors (January–December 2019). Circulating microvesicles (MVs) of different cellular origins (platelet, erythrocyte, and endothelial) were measured by a standardized flow cytometry protocol as novel markers of vascular injury and pro-coagulant activity. (3) Results: we recruited 45 survivors after a median of 2.3 (range 1.1–13.2) years from alloHCT, and 45 controls. The majority of patients suffered from acute (44%) and/or chronic (66%) graft-versus-host disease (GVHD). Although the two groups were matched for traditional cardiovascular risk factors, alloHCT survivors showed significantly increased platelet and erythrocyte MVs compared to controls. Within alloHCT survivors, erythrocyte MVs were significantly increased in patients with a previous history of thrombotic microangiopathy. Interestingly, endothelial MVs were significantly increased only in alloHCT recipients of a myeloablative conditioning. Furthermore, MVs of different origins showed a positive association with each other. (4) Conclusions: endothelial dysfunction and increased thrombotic risk are evident in alloHCT recipients long after alloHCT, independently of traditional cardiovascular risk factors. An apparent synergism of these pathophysiological processes may be strongly involved in the subsequent establishment of cardiovascular disease. View Full-Text
Keywords: allogeneic hematopoietic cell transplantation; vascular injury; pro-coagulant activity; microvesicles allogeneic hematopoietic cell transplantation; vascular injury; pro-coagulant activity; microvesicles
MDPI and ACS Style

Gavriilaki, E.; Sakellari, I.; Anyfanti, P.; Batsis, I.; Vardi, A.; Bousiou, Z.; Lazaridis, A.; Nikolaidou, B.; Zarifis, I.; Masmanidou, M.; Yiannaki, E.; Markala, D.; Anagnostopoulos, A.; Douma, S.; Gkaliagkousi, E. Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation. Int. J. Mol. Sci. 2020, 21, 9768. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21249768

AMA Style

Gavriilaki E, Sakellari I, Anyfanti P, Batsis I, Vardi A, Bousiou Z, Lazaridis A, Nikolaidou B, Zarifis I, Masmanidou M, Yiannaki E, Markala D, Anagnostopoulos A, Douma S, Gkaliagkousi E. Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation. International Journal of Molecular Sciences. 2020; 21(24):9768. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21249768

Chicago/Turabian Style

Gavriilaki, Eleni, Ioanna Sakellari, Panagiota Anyfanti, Ioannis Batsis, Anna Vardi, Zoi Bousiou, Antonios Lazaridis, Barbara Nikolaidou, Ippokratis Zarifis, Marianna Masmanidou, Efthalia Yiannaki, Dimitra Markala, Achilles Anagnostopoulos, Stella Douma, and Eugenia Gkaliagkousi. 2020. "Assessment of Endothelial Injury and Pro-Coagulant Activity Using Circulating Microvesicles in Survivors of Allogeneic Hematopoietic Cell Transplantation" International Journal of Molecular Sciences 21, no. 24: 9768. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21249768

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