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Article

Genotoxic Properties of Synthetic Cannabinoids on TK6 Human Cells by Flow Cytometry

1
Department of Pharmacy and Biotechnology, Alma Mater Studiorum University of Bologna, 40126 Bologna, Italy
2
Department of Morphology, Experimental Medicine and Surgery, Section of Legal Medicine and LTTA Center, University of Ferrara, 44121 Ferrara, Italy
3
Collaborative Center for the Italian National Early Warning System, Department of Anti-Drug Policies, Presidency of the Council of Ministers, 44121 Ferrara, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 1150; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031150
Received: 28 November 2019 / Revised: 23 January 2020 / Accepted: 5 February 2020 / Published: 9 February 2020
(This article belongs to the Section Molecular Toxicology)
Novel Psychoactive Substances (NPS) include several classes of substances such as synthetic cannabinoids (SCBs), an emerging alternative to marijuana, easily purchasable on internet. SCBs are more dangerous than Δ9-Tetrahydrocannabinol as a consequence of their stronger affinities for the CB1 and CB2 receptors, which may result in longer duration of distinct effects, greater potency, and toxicity. The information on SCBs cytotoxicity, genotoxicity, mutagenicity, and long-term effects is scarce. This fact suggests the urgent need to increase available data and to investigate if some SCBs have an impact on the stability of genetic material. Therefore, the aim of the present study was the evaluation of the mutagenic effect of different SCBs belonging to indole- and indazole-structures. The analyzes were conducted in vitro on human TK6 cells and mutagenicity were measured as micronucleus fold increase by flow cytometry. Our results have highlighted, for the first time, the mutagenic capacity of four SCBs, in particular in terms of chromosomal damage induction. We underline the serious potential toxicity of SCBs that suggests the need to proceed with the studies of other different synthetic compounds. Moreover, we identified a method that allows a rapid but effective screening of NPS placed on the market increasingly faster. View Full-Text
Keywords: synthetic cannabinoids; 5F-AKB-48; APINAC; STS-135; JWH-018-CL; JWH-018; BB-22; genotoxicity; mutagenicity; flow cytometry synthetic cannabinoids; 5F-AKB-48; APINAC; STS-135; JWH-018-CL; JWH-018; BB-22; genotoxicity; mutagenicity; flow cytometry
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MDPI and ACS Style

Lenzi, M.; Cocchi, V.; Cavazza, L.; Bilel, S.; Hrelia, P.; Marti, M. Genotoxic Properties of Synthetic Cannabinoids on TK6 Human Cells by Flow Cytometry. Int. J. Mol. Sci. 2020, 21, 1150. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031150

AMA Style

Lenzi M, Cocchi V, Cavazza L, Bilel S, Hrelia P, Marti M. Genotoxic Properties of Synthetic Cannabinoids on TK6 Human Cells by Flow Cytometry. International Journal of Molecular Sciences. 2020; 21(3):1150. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031150

Chicago/Turabian Style

Lenzi, Monia, Veronica Cocchi, Luca Cavazza, Sabrine Bilel, Patrizia Hrelia, and Matteo Marti. 2020. "Genotoxic Properties of Synthetic Cannabinoids on TK6 Human Cells by Flow Cytometry" International Journal of Molecular Sciences 21, no. 3: 1150. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031150

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