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Article

Testosterone Promotes the Proliferation of Chicken Embryonic Myoblasts Via Androgen Receptor Mediated PI3K/Akt Signaling Pathway

1
Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China
2
National Experimental Teaching Demonstration Center for Animal Science, Nanjing Agricultural University, Nanjing 210095, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 1152; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031152
Received: 31 December 2019 / Revised: 3 February 2020 / Accepted: 6 February 2020 / Published: 9 February 2020
(This article belongs to the Section Molecular Biology)
Testosterone (T) is essential for muscle fiber formation and growth. However, the specific mechanism by which T regulates skeletal muscle development in chicken embryos remains unclear. In this study, the role of T in myoblast proliferation both in vivo and in vitro was investigated. Results showed that the T administration significantly increased the ratio of breast muscle and leg muscle. T induced a significant increase in the cross-sectional area (CSA) and density of myofiber and the ratio of PAX7-positive cells in the skeletal muscle. Exogenous T also induced the upregulation of myogenic regulatory factors (MRFs) and cyclin-dependent kinases (CDK2)/Cyclin D1 (CCND1) and protein levels of androgen receptor (AR), p-Akt and PAX7. Furthermore, T treatment significantly promoted myoblasts cultured in vitro entering a new cell cycle and increased PAX7-positive cells. The mRNA and protein expression of AR and PAX7 were upregulated when treated with T compared to that of the control. The addition of T induced proliferation accompanied by increasing AR level as well as PI3K (Phosphoinositide 3-kinase)/Akt activation. However, T-induced proliferation was attenuated by AR, PI3K, and Akt-specific inhibitors. These data indicated that the pro-proliferative effect of T was regulated though AR in response to the activation of PI3K/Akt signalling pathway. View Full-Text
Keywords: chicken; myoblasts; PI3K/Akt pathway; proliferation; testosterone chicken; myoblasts; PI3K/Akt pathway; proliferation; testosterone
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MDPI and ACS Style

Li, D.; Wang, Q.; Shi, K.; Lu, Y.; Yu, D.; Shi, X.; Du, W.; Yu, M. Testosterone Promotes the Proliferation of Chicken Embryonic Myoblasts Via Androgen Receptor Mediated PI3K/Akt Signaling Pathway. Int. J. Mol. Sci. 2020, 21, 1152. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031152

AMA Style

Li D, Wang Q, Shi K, Lu Y, Yu D, Shi X, Du W, Yu M. Testosterone Promotes the Proliferation of Chicken Embryonic Myoblasts Via Androgen Receptor Mediated PI3K/Akt Signaling Pathway. International Journal of Molecular Sciences. 2020; 21(3):1152. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031152

Chicago/Turabian Style

Li, Dongfeng, Qin Wang, Kai Shi, Yinglin Lu, Debing Yu, Xiaoli Shi, Wenxing Du, and Minli Yu. 2020. "Testosterone Promotes the Proliferation of Chicken Embryonic Myoblasts Via Androgen Receptor Mediated PI3K/Akt Signaling Pathway" International Journal of Molecular Sciences 21, no. 3: 1152. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031152

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