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Article

An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification

1
Instituto de Biología Molecular y Celular de Plantas, Universidad Politécnica de Valencia-Consejo Superior de Investigaciones Científicas, Camino de Vera, 46022 Valencia, Spain
2
Departament de Biologia Vegetal, Facultat de Farmàcia, Universitat de València, 46100 València, Spain
3
Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, 29071 Málaga, Spain
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(3), 1173; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031173
Received: 30 January 2020 / Revised: 4 February 2020 / Accepted: 8 February 2020 / Published: 10 February 2020
(This article belongs to the Special Issue ROS and Abiotic Stress in Plants)
Intracellular acid stress inhibits plant growth by unknown mechanisms and it occurs in acidic soils and as consequence of other stresses. In order to identify mechanisms of acid toxicity, we screened activation-tagging lines of Arabidopsis thaliana for tolerance to intracellular acidification induced by organic acids. A dominant mutant, sbt4.13-1D, was isolated twice and shown to over-express subtilase SBT4.13, a protease secreted into endoplasmic reticulum. Activity measurements and immuno-detection indicate that the mutant contains less plasma membrane H+-ATPase (PMA) than wild type, explaining the small size, electrical depolarization and decreased cytosolic pH of the mutant but not organic acid tolerance. Addition of acetic acid to wild-type plantlets induces production of ROS (Reactive Oxygen Species) measured by dichlorodihydrofluorescein diacetate. Acid-induced ROS production is greatly decreased in sbt4.13-1D and atrboh-D,F mutants. The latter is deficient in two major NADPH oxidases (NOXs) and is tolerant to organic acids. These results suggest that intracellular acidification activates NOXs and the resulting oxidative stress is important for inhibition of growth. The inhibition of acid-activated NOXs in the sbt4.13-1D mutant compensates inhibition of PMA to increase acid tolerance. View Full-Text
Keywords: activation-tagging; organic acids; H+-ATPase; NADPH oxidase; ROS activation-tagging; organic acids; H+-ATPase; NADPH oxidase; ROS
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MDPI and ACS Style

Bissoli, G.; Muñoz-Bertomeu, J.; Bueso, E.; Sayas, E.; Vilcara, E.A.; Felipo, A.; Niñoles, R.; Rubio, L.; Fernández, J.A.; Serrano, R. An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification. Int. J. Mol. Sci. 2020, 21, 1173. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031173

AMA Style

Bissoli G, Muñoz-Bertomeu J, Bueso E, Sayas E, Vilcara EA, Felipo A, Niñoles R, Rubio L, Fernández JA, Serrano R. An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification. International Journal of Molecular Sciences. 2020; 21(3):1173. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031173

Chicago/Turabian Style

Bissoli, Gaetano, Jesús Muñoz-Bertomeu, Eduardo Bueso, Enric Sayas, Edgardo A. Vilcara, Amelia Felipo, Regina Niñoles, Lourdes Rubio, José A. Fernández, and Ramón Serrano. 2020. "An Arabidopsis Mutant Over-Expressing Subtilase SBT4.13 Uncovers the Role of Oxidative Stress in the Inhibition of Growth by Intracellular Acidification" International Journal of Molecular Sciences 21, no. 3: 1173. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21031173

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