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Article

The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer

1
Departments of Medicine and Biomedical Engineering, College of Medicine, University of Arizona, Tucson, AZ 85724, USA
2
Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milan 20133, Italy
3
Divisione di Medicina, Ospedale San Paolo, Dipartimento di Scienze della Salute, Università degli Studi di Milano, Milan 20122, Italy
4
Università Vita Salute, San Raffaele, Milan 20132, Italy
*
Author to whom correspondence should be addressed.
Both first authors contributed equally to this manuscript.
Both authors served as senior authors for this work.
Int. J. Mol. Sci. 2020, 21(4), 1174; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041174
Received: 21 December 2019 / Revised: 7 February 2020 / Accepted: 7 February 2020 / Published: 11 February 2020
(This article belongs to the Special Issue Molecular Research on Platelet Activity in Health and Disease)
As key cellular elements of hemostasis, platelets represent a primary target for thrombosis and bleeding management. Currently, therapeutic manipulations of platelet function (antithrombotic drugs) and count (platelet transfusion) are performed with limited or no real-time monitoring of the desired outcome at the point-of-care. To address the need, we have designed and fabricated an easy-to-use, accurate, and portable impedance aggregometer called “MICELI” (MICrofluidic, ELectrical, Impedance). It improves on current platelet aggregation technology by decreasing footprint, assay complexity, and time to obtain results. The current study aimed to optimize the MICELI protocol; validate sensitivity to aggregation agonists and key blood parameters, i.e., platelet count and hematocrit; and verify the MICELI operational performance as compared to commercial impedance aggregometry. We demonstrated that the MICELI aggregometer could detect platelet aggregation in 250 μL of whole blood or platelet-rich plasma, stimulated by ADP, TRAP-6, collagen, epinephrine, and calcium ionophore. Using hirudin as blood anticoagulant allowed higher aggregation values. Aggregation values obtained by the MICELI strongly correlated with platelet count and were not affected by hematocrit. The operational performance comparison of the MICELI and the Multiplate® Analyzer demonstrated strong correlation and similar interdonor distribution of aggregation values obtained between these devices. With the proven reliability of the data obtained by the MICELI aggregometer, it can be further translated into a point-of-care diagnostic device aimed at monitoring platelet function in order to guide pharmacological hemostasis management and platelet transfusions. View Full-Text
Keywords: platelet aggregation; electrical impedance; whole blood aggregometry; point-of-care; platelet function testing platelet aggregation; electrical impedance; whole blood aggregometry; point-of-care; platelet function testing
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MDPI and ACS Style

Roka-Moiia, Y.; Bozzi, S.; Ferrari, C.; Mantica, G.; Dimasi, A.; Rasponi, M.; Santoleri, A.; Scavone, M.; Consolo, F.; Cattaneo, M.; Slepian, M.J.; Redaelli, A. The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer. Int. J. Mol. Sci. 2020, 21, 1174. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041174

AMA Style

Roka-Moiia Y, Bozzi S, Ferrari C, Mantica G, Dimasi A, Rasponi M, Santoleri A, Scavone M, Consolo F, Cattaneo M, Slepian MJ, Redaelli A. The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer. International Journal of Molecular Sciences. 2020; 21(4):1174. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041174

Chicago/Turabian Style

Roka-Moiia, Yana, Silvia Bozzi, Chiara Ferrari, Gabriele Mantica, Annalisa Dimasi, Marco Rasponi, Andrea Santoleri, Mariangela Scavone, Filippo Consolo, Marco Cattaneo, Marvin J. Slepian, and Alberto Redaelli. 2020. "The MICELI (MICrofluidic, ELectrical, Impedance): Prototyping a Point-of-Care Impedance Platelet Aggregometer" International Journal of Molecular Sciences 21, no. 4: 1174. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041174

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