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The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing

1
College of Life Science, Jiangsu Normal University, Xuzhou 221116, China
2
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University, Beijing100048, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(4), 1518; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041518
Received: 8 February 2020 / Revised: 20 February 2020 / Accepted: 21 February 2020 / Published: 23 February 2020
(This article belongs to the Section Bioactives and Nutraceuticals)
Perillaldehyde (PAE), an essential oil in Perilla plants, serves as a safe flavor ingredient in foods, and shows an effectively antifungal activity. Reactive oxygen species (ROS) accumulation in Aspergillus flavus plays a critical role in initiating a metacaspase-dependent apoptosis. However, the reason for ROS accumulation in A. flavus is not yet clear. Using transcriptome sequencing of A. flavus treated with different concentrations of PAE, our data showed that the ROS accumulation might have been as a result of an inhibition of energy metabolism with less production of reducing power. By means of GO and KEGG enrichment analysis, we screened four key pathways, which were divided into two distinct groups: a downregulated group that was made up of the glycolysis and pentose phosphate pathway, and an upregulated group that consisted of MAPK signaling pathway and GSH metabolism pathway. The inhibition of dehydrogenase gene expression in two glycometabolism pathways might play a crucial role in antifungal mechanism of PAE. Also, in our present study, we systematically showed a gene interaction network of how genes of four subsets are effected by PAE stress on glycometabolism, oxidant damage repair, and cell cycle control. This research may contribute to explaining an intrinsic antifungal mechanism of PAE against A. flavus. View Full-Text
Keywords: Aspergillus flavus; Perilla; oxidative stress; energy metabolism; antifungal mechanism Aspergillus flavus; Perilla; oxidative stress; energy metabolism; antifungal mechanism
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MDPI and ACS Style

Pan, C.; Li, Y.-X.; Yang, K.; Famous, E.; Ma, Y.; He, X.; Geng, Q.; Liu, M.; Tian, J. The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing. Int. J. Mol. Sci. 2020, 21, 1518. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041518

AMA Style

Pan C, Li Y-X, Yang K, Famous E, Ma Y, He X, Geng Q, Liu M, Tian J. The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing. International Journal of Molecular Sciences. 2020; 21(4):1518. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041518

Chicago/Turabian Style

Pan, Chao, Yong-Xin Li, Kunlong Yang, Erhunmwunsee Famous, Yan Ma, Xiaona He, Qingru Geng, Man Liu, and Jun Tian. 2020. "The Molecular Mechanism of Perillaldehyde Inducing Cell Death in Aspergillus flavus by Inhibiting Energy Metabolism Revealed by Transcriptome Sequencing" International Journal of Molecular Sciences 21, no. 4: 1518. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041518

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