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Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids

State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
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Int. J. Mol. Sci. 2020, 21(4), 1521; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041521
Received: 19 December 2019 / Revised: 20 February 2020 / Accepted: 20 February 2020 / Published: 23 February 2020
(This article belongs to the Section Molecular Plant Sciences)
The leaf blade is the main photosynthetic organ and its morphology is related to light energy capture and conversion efficiency. We isolated a novel rice Dynamic Narrow-Rolled Leaf 1 (dnrl1) mutant showing reduced width of leaf blades, rolled leaves and lower chlorophyll content. The narrow-rolled leaf phenotype resulted from the reduced number of small longitudinal veins per leaf, smaller size and irregular arrangement of bulliform cells compared with the wild-type. DNRL1 was mapped to chromosome 7 and encoded a putative 3-deoxy-7-phosphoheptulonate synthase (DAHPS) which catalyzes the conversion of phosphoenolpyruvate and D-erythrose 4-phosphate to DAHP and phosphate. Sequence analysis revealed that a single base substitution (T–A) was detected in dnrl1, leading to a single amino acid change (L376H) in the coding protein. The mutation led to a lower expression level of DNRL1 as well as the lower activity of DAHPS in the mutant compared with the wild type. Genetic complementation and over-expression of DNRL1 could rescue the narrow-rolled phenotype. DNRL1 was constitutively expressed in all tested organs and exhibited different expression patterns from other narrow-rolled leaf genes. DNRL1-GFP located to chloroplasts. The lower level of chlorophyll in dnrl1 was associated with the downregulation of the genes responsible for chlorophyll biosynthesis and photosynthesis. Furthermore, dnrl1 showed significantly reduced levels of aromatic amino acids including Trp, Phe and Tyr. We conclude that OsDAHPS, encoded by DNRL1, plays a critical role in leaf morphogenesis by mediating the biosynthesis of amino acids in rice. View Full-Text
Keywords: narrow-rolled leaf; DAHPS; map-based cloning; rice (Oryza sativa L.) narrow-rolled leaf; DAHPS; map-based cloning; rice (Oryza sativa L.)
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MDPI and ACS Style

Wang, H.; Shi, Y.; Zhang, X.; Xu, X.; Wu, J.-L. Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids. Int. J. Mol. Sci. 2020, 21, 1521. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041521

AMA Style

Wang H, Shi Y, Zhang X, Xu X, Wu J-L. Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids. International Journal of Molecular Sciences. 2020; 21(4):1521. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041521

Chicago/Turabian Style

Wang, Huimei, Yongfeng Shi, Xiaobo Zhang, Xia Xu, and Jian-Li Wu. 2020. "Characterization of a Novel Rice Dynamic Narrow-Rolled Leaf Mutant with Deficiencies in Aromatic Amino Acids" International Journal of Molecular Sciences 21, no. 4: 1521. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21041521

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