Next Article in Journal
Histone Deacetylation Inhibitors as Modulators of Regulatory T Cells
Next Article in Special Issue
Health Impact of Silver Nanoparticles: A Review of the Biodistribution and Toxicity Following Various Routes of Exposure
Previous Article in Journal
Hypergravity Activates a Pro-Angiogenic Homeostatic Response by Human Capillary Endothelial Cells
Previous Article in Special Issue
Differential Cytotoxicity Induced by Transition Metal Oxide Nanoparticles is a Function of Cell Killing and Suppression of Cell Proliferation
Article

Cytotoxicity of NiO and Ni(OH)2 Nanoparticles Is Mediated by Oxidative Stress-Induced Cell Death and Suppression of Cell Proliferation

1
Department of Biological Sciences, Missouri University of Science and Technology, Rolla, MO 65409-1120, USA
2
Department of Natural Resources and Environmental Studies, National Dong Hwa University, Hualien 97401, Taiwan
3
Department of Chemistry, Middle Tennessee State University, Murfreesboro, TN 37132, USA
4
Department of Biomedical Sciences, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA
*
Author to whom correspondence should be addressed.
M.H.C. and N.J.H. contributed equally.
Int. J. Mol. Sci. 2020, 21(7), 2355; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072355
Received: 12 February 2020 / Revised: 21 March 2020 / Accepted: 26 March 2020 / Published: 28 March 2020
(This article belongs to the Special Issue Nanotoxicology and Nanosafety 2.0)
The use of nanomaterial-based products continues to grow with advancing technology. Understanding the potential toxicity of nanoparticles (NPs) is important to ensure that products containing them do not impose harmful effects to human or environmental health. In this study, we evaluated the comparative cytotoxicity between nickel oxide (NiO) and nickel hydroxide (Ni(OH)2) in human bronchoalveolar carcinoma (A549) and human hepatocellular carcinoma (HepG2) cell lines. Cellular viability studies revealed cell line-specific cytotoxicity in which nickel NPs were toxic to A549 cells but relatively nontoxic to HepG2 cells. Time-, concentration-, and particle-specific cytotoxicity was observed in A549 cells. NP-induced oxidative stress triggered dissipation of mitochondrial membrane potential and induction of caspase-3 enzyme activity. The subsequent apoptotic events led to reduction in cell number. In addition to cell death, suppression of cell proliferation played an essential role in regulating cell number. Collectively, the observed cell viability is a function of cell death and suppression of proliferation. Physical and chemical properties of NPs such as total surface area and metal dissolution are in agreement with the observed differential cytotoxicity. Understanding the properties of NPs is essential in informing the design of safer materials. View Full-Text
Keywords: nanoparticles; viability; cell proliferation; physicochemical properties; oxidative stress; caspase-3; mitochondrial membrane potential; cell cycle; apoptosis nanoparticles; viability; cell proliferation; physicochemical properties; oxidative stress; caspase-3; mitochondrial membrane potential; cell cycle; apoptosis
Show Figures

Graphical abstract

MDPI and ACS Style

Cambre, M.H.; Holl, N.J.; Wang, B.; Harper, L.; Lee, H.-J.; Chusuei, C.C.; Hou, F.Y.S.; Williams, E.T.; Argo, J.D.; Pandey, R.R.; Huang, Y.-W. Cytotoxicity of NiO and Ni(OH)2 Nanoparticles Is Mediated by Oxidative Stress-Induced Cell Death and Suppression of Cell Proliferation. Int. J. Mol. Sci. 2020, 21, 2355. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072355

AMA Style

Cambre MH, Holl NJ, Wang B, Harper L, Lee H-J, Chusuei CC, Hou FYS, Williams ET, Argo JD, Pandey RR, Huang Y-W. Cytotoxicity of NiO and Ni(OH)2 Nanoparticles Is Mediated by Oxidative Stress-Induced Cell Death and Suppression of Cell Proliferation. International Journal of Molecular Sciences. 2020; 21(7):2355. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072355

Chicago/Turabian Style

Cambre, Melissa H., Natalie J. Holl, Bolin Wang, Lucas Harper, Han-Jung Lee, Charles C. Chusuei, Fang Y.S. Hou, Ethan T. Williams, Jerry D. Argo, Raja R. Pandey, and Yue-Wern Huang. 2020. "Cytotoxicity of NiO and Ni(OH)2 Nanoparticles Is Mediated by Oxidative Stress-Induced Cell Death and Suppression of Cell Proliferation" International Journal of Molecular Sciences 21, no. 7: 2355. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072355

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop