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Article

Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma

1
Department of Medical Research, MacKay Memorial Hospital, Taipei 10449, Taiwan
2
Department of Oral and Maxillofacial Surgery, MacKay Memorial Hospital, Taipei 10449, Taiwan
3
Institute of Oral Biology, School of Dentistry, National Yang-Ming University, Taipei 11221, Taiwan
4
School of Dentistry, National Yang-Ming University, Taipei 11221, Taiwan
5
Department of Stomatology, Veterans General Hospital, Taipei 11217, Taiwan
6
Department of Stomatology, Medical Education and Research, Veterans General Hospital, Taipei 11217, Taiwan
*
Authors to whom correspondence should be addressed.
The contributions of these two authors are equal.
Int. J. Mol. Sci. 2020, 21(7), 2360; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072360
Received: 1 March 2020 / Revised: 25 March 2020 / Accepted: 26 March 2020 / Published: 29 March 2020
(This article belongs to the Special Issue Oral Fibrosis and Oral Cancer: From Molecular Targets to Therapeutics)
Oral squamous cell carcinoma (OSCC) is a cancerous disease with poor prognosis. According to the statistics, the 5-year survival rate has not improved significantly over the past 20 years. The platelet-derived growth factor (PDGF) and its signaling pathway is a key regulator of angiogenesis and tumorigenesis. High level of PDGF and its receptor (PDGFR) have been reported in several types of malignancies. In this study, we investigated the relationship of the molecular expression levels of PDGF and PDGFR with clinicopathological parameters in OSCC. To this end, we measured the mRNA and protein levels of PDGF and PDGFR by real-time quantitative PCR (qRT-PCR), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA), respectively. We found positive correlations of the mRNA levels of PDGFA, PDGFB, and PDGFRB with lymph node metastasis and poor overall survival (OS). High expression of PDGF, PDGFRA, and PDGFRB were remarkably associated with lymph node metastasis and poor OS, as determined by immunohistochemistry. Preoperative serum levels of PDGF-AA and PDGF-BB had a positive correlation with preoperative platelet count. Elevated serum levels of PDGF-AA. PDGF-BB, and platelet count correlated with lymph node metastasis and an unfavorable outcome. In multivariate Cox regression analysis, PDGFA mRNA, PDGFB mRNA, PDGFRB mRNA, PDGF immunoexpression, PDGFRB immunoexpression, serum PDGF-AA, serum PDGF-BB, and platelet count emerged as significant independent prognostic factors for OS. In vitro, we found that elevated PDGF promotes colony formation, migration, and invasiveness of SAS and OECM-1 cancer cell lines. Our results suggest that the expression level of serum PDGF has the potential to become a useful diagnostic marker for the prognosis of OSCC. In addition, PDGFR should be considered as a potential therapeutic target for OSCC. Furthermore, research should be undertaken to elucidate the role of PDGF and PDGFR regarding the behavior of tumor cells in OSCC. View Full-Text
Keywords: oral squamous cell carcinoma; platelet-derived growth factor; platelet-derived growth factor receptor; survival oral squamous cell carcinoma; platelet-derived growth factor; platelet-derived growth factor receptor; survival
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MDPI and ACS Style

Lin, L.-H.; Lin, J.-S.; Yang, C.-C.; Cheng, H.-W.; Chang, K.-W.; Liu, C.-J. Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma. Int. J. Mol. Sci. 2020, 21, 2360. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072360

AMA Style

Lin L-H, Lin J-S, Yang C-C, Cheng H-W, Chang K-W, Liu C-J. Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma. International Journal of Molecular Sciences. 2020; 21(7):2360. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072360

Chicago/Turabian Style

Lin, Li-Han, Jiun-Sheng Lin, Cheng-Chieh Yang, Hui-Wen Cheng, Kuo-Wei Chang, and Chung-Ji Liu. 2020. "Overexpression of Platelet-Derived Growth Factor and Its Receptor Are Correlated with Oral Tumorigenesis and Poor Prognosis in Oral Squamous Cell Carcinoma" International Journal of Molecular Sciences 21, no. 7: 2360. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072360

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