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Article

Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici

1
College of Life Sciences, Qingdao University, Qingdao 266071, China
2
College of Life Sciences, Capital Normal University, Beijing 100084, China
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(7), 2640; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072640
Received: 25 February 2020 / Revised: 5 April 2020 / Accepted: 8 April 2020 / Published: 10 April 2020
(This article belongs to the Special Issue Wheat and Barley: Acclimatization to Abiotic and Biotic Stress)
Powdery mildew disease caused by Blumeria graminis f.sp. tritici (Bgt) leads to severe economic losses in bread wheat (Triticum aestivum L.). To date, only a few epigenetic modulators have been revealed to regulate wheat powdery mildew resistance. In this study, the histone deacetylase 2 (HD2) type histone deacetylase TaHDT701 was identified as a negative regulator of wheat defense responses to Bgt. Using multiple approaches, we demonstrated that TaHDT701 associates with the RPD3 type histone deacetylase TaHDA6 and the WD40-repeat protein TaHOS15 to constitute a histone deacetylase complex, in which TaHDT701 could stabilize the TaHDA6-TaHOS15 association. Furthermore, knockdown of TaHDT701, TaHDA6, and TaHOS15 resulted in enhanced wheat powdery mildew resistance, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex negatively regulates wheat defense responses to Bgt. Moreover, chromatin immunoprecipitation assays revealed that TaHDT701 could function in concert with TaHOS15 to recruit TaHDA6 to the promoters of defense-related genes such as TaPR1, TaPR2, TaPR5, and TaWRKY45. In addition, silencing of TaHDT701, TaHDA6, and TaHOS15 resulted in the up-regulation of TaPR1, TaPR2, TaPR5, and TaWRKY45 accompanied with increased histone acetylation and methylation, as well as reduced nucleosome occupancy, at their promoters, suggesting that the TaHDT701-TaHDA6-TaHOS15 histone deacetylase complex suppresses wheat powdery mildew resistance by modulating chromatin state at defense-related genes. View Full-Text
Keywords: HDT701; HDA6; histone modification; wheat; Blumeria graminis f.sp. tritici HDT701; HDA6; histone modification; wheat; Blumeria graminis f.sp. tritici
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MDPI and ACS Style

Zhi, P.; Kong, L.; Liu, J.; Zhang, X.; Wang, X.; Li, H.; Sun, M.; Li, Y.; Chang, C. Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici. Int. J. Mol. Sci. 2020, 21, 2640. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072640

AMA Style

Zhi P, Kong L, Liu J, Zhang X, Wang X, Li H, Sun M, Li Y, Chang C. Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici. International Journal of Molecular Sciences. 2020; 21(7):2640. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072640

Chicago/Turabian Style

Zhi, Pengfei; Kong, Lingyao; Liu, Jiao; Zhang, Xiaona; Wang, Xiaoyu; Li, Haoyu; Sun, Maokai; Li, Yan; Chang, Cheng. 2020. "Histone Deacetylase TaHDT701 Functions in TaHDA6-TaHOS15 Complex to Regulate Wheat Defense Responses to Blumeria graminis f.sp. tritici" Int. J. Mol. Sci. 21, no. 7: 2640. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072640

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