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Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA

Department of Botany and Nature Protection, University of Warmia and Mazury in Olsztyn, Plac Łódzki 1, 10-727 Olsztyn, Poland
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Int. J. Mol. Sci. 2020, 21(7), 2645; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072645
Received: 20 March 2020 / Revised: 6 April 2020 / Accepted: 9 April 2020 / Published: 10 April 2020
(This article belongs to the Section Molecular Microbiology)
Fungi and oomycetes encompass many pathogens affecting crops worldwide. Their effective control requires screening pathogens across the local and international trade networks along with the monitoring of pathogen inocula in the field. Fundamentals to all of these concerns are their efficient detection, identification, and quantification. The use of molecular markers showed the best promise in the field of plant pathogen diagnostics. However, despite the unquestionable benefits of DNA-based methods, two significant limitations are associated with their use. The first limitation concerns the insufficient level of sensitivity due to the very low and uneven distribution of pathogens in plant material. The second limitation pertains to the inability of widely used diagnostic assays to detect cryptic species. Targeting mtDNA appears to provide a solution to these challenges. Its high copy number in microbial cells makes mtDNA an attractive target for developing highly sensitive assays. In addition, previous studies on different pathogen taxa indicated that mitogenome sequence variation could improve cryptic species delimitation accuracy. This review sheds light on the potential application of mtDNA for pathogen diagnostics. This paper covers a brief description of qPCR and DNA barcoding as two major strategies enabling the diagnostics of plant pathogenic fungi and oomycetes. Both strategies are discussed along with the potential use of mtDNA, including their strengths and weaknesses. View Full-Text
Keywords: plant pathogenic fungi and oomycetes; detection; identification; quantification; DNA-based markers; mitochondrial DNA plant pathogenic fungi and oomycetes; detection; identification; quantification; DNA-based markers; mitochondrial DNA
MDPI and ACS Style

Kulik, T.; Bilska, K.; Żelechowski, M. Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA. Int. J. Mol. Sci. 2020, 21, 2645. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072645

AMA Style

Kulik T, Bilska K, Żelechowski M. Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA. International Journal of Molecular Sciences. 2020; 21(7):2645. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072645

Chicago/Turabian Style

Kulik, Tomasz; Bilska, Katarzyna; Żelechowski, Maciej. 2020. "Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA" Int. J. Mol. Sci. 21, no. 7: 2645. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072645

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