Next Article in Journal
Circulating Tumor Cell Clusters: United We Stand Divided We Fall
Previous Article in Journal
Karyopherin α-2 Mediates MDC1 Nuclear Import through a Functional Nuclear Localization Signal in the tBRCT Domain of MDC1
Article

Functional Analysis of IRF1 Reveals its Role in the Activation of the Type I IFN Pathway in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)

by 1,2, 1,2, 1,2, 1,3, 1,3, 1,3,4 and 1,2,3,4,*
1
Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural Affairs, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
2
Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed Industry, Guangzhou 510300, China
3
Tropical Aquaculture Research and Development Center, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China
4
Sanya Tropical Fisheries Research Institute, Sanya 510300, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(7), 2652; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072652
Received: 4 March 2020 / Revised: 1 April 2020 / Accepted: 9 April 2020 / Published: 10 April 2020
(This article belongs to the Section Molecular Biology)
Interferon (IFN) regulatory factor 1 (IRF1), a transcription factor with a novel helix–turn–helix DNA-binding domain, plays a crucial role in innate immunity by regulating the type I IFN signaling pathway. However, the regulatory mechanism through which IRF1 regulates type I IFN in fish is not yet elucidated. In the present study, IRF1 was characterized from golden pompano, Trachinotus ovatus (designated ToIRF1), and its immune function was identified to elucidate the transcriptional regulatory mechanism of ToIFNa3. The full-length complementary DNA (cDNA) of IRF1 is 1763 bp, including a 900-bp open reading frame (ORF) encoding a 299-amino-acid polypeptide. The putative protein sequence has 42.7–71.7% identity to fish IRF1 and possesses a representative conserved domain (a DNA-binding domain (DBD) at the N-terminus). The genomic DNA sequence of ToIRF1 consists of eight exons and seven introns. Moreover, ToIRF1 is constitutively expressed in all examined tissues, with higher levels being observed in immune-relevant tissues (whole blood, gill, and skin). Additionally, Cryptocaryon irritans challenge in vivo increases ToIRF1 expression in the skin as determined by Western blotting (WB); however, protein levels of ToIRF1 in the gill did not change significantly. The subcellular localization indicates that ToIRF1 is localized in the nucleus and cytoplasm with or without polyinosinic/polycytidylic acid (poly (I:C)) induction. Furthermore, overexpression of ToIRF1 or ToIFNa3 shows that ToIRF1 can notably activate ToIFNa3 and interferon signaling molecule expression. Promoter sequence analysis finds that several interferon stimulating response element (ISRE) binding sites are present in the promoter of ToIFNa3. Additionally, truncation, point mutation, and electrophoretic mobile shift (EMSA) assays confirmed that ToIRF1 M5 ISRE binding sites are functionally important for ToIFNa3 transcription. These results may help to illuminate the roles of teleost IRF1 in the transcriptional mechanisms of type I IFN in the immune process. View Full-Text
Keywords: Trachinotus ovatus; IRF1; type I IFN; mutation analyses; EMSA Trachinotus ovatus; IRF1; type I IFN; mutation analyses; EMSA
Show Figures

Figure 1

MDPI and ACS Style

Zhu, K.-C.; Zhang, N.; Liu, B.-S.; Guo, L.; Guo, H.-Y.; Jiang, S.-G.; Zhang, D.-C. Functional Analysis of IRF1 Reveals its Role in the Activation of the Type I IFN Pathway in Golden Pompano, Trachinotus ovatus (Linnaeus 1758). Int. J. Mol. Sci. 2020, 21, 2652. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072652

AMA Style

Zhu K-C, Zhang N, Liu B-S, Guo L, Guo H-Y, Jiang S-G, Zhang D-C. Functional Analysis of IRF1 Reveals its Role in the Activation of the Type I IFN Pathway in Golden Pompano, Trachinotus ovatus (Linnaeus 1758). International Journal of Molecular Sciences. 2020; 21(7):2652. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072652

Chicago/Turabian Style

Zhu, Ke-Cheng; Zhang, Nan; Liu, Bao-Suo; Guo, Liang; Guo, Hua-Yang; Jiang, Shi-Gui; Zhang, Dian-Chang. 2020. "Functional Analysis of IRF1 Reveals its Role in the Activation of the Type I IFN Pathway in Golden Pompano, Trachinotus ovatus (Linnaeus 1758)" Int. J. Mol. Sci. 21, no. 7: 2652. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21072652

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Search more from Scilit
 
Search
Back to TopTop