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Cystic Fibrosis, CFTR, and Colorectal Cancer
Communication

Mechanical Properties of Human Bronchial Epithelial Cells Expressing Wt- and Mutant CFTR

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BioISI – Instituto de Biosistemas e Ciências Integrativas, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
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Departamento de Física, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
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Departamento de Química e Bioquímica, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 2916; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082916
Received: 10 March 2020 / Revised: 15 April 2020 / Accepted: 16 April 2020 / Published: 21 April 2020
(This article belongs to the Special Issue Therapeutic Approaches for Cystic Fibrosis)
Cystic fibrosis (CF) is caused by mutations in the gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR). A single recessive mutation, the deletion of phenylalanine 508 (F508del), causes severe CF and resides on 70% of mutant chromosomes. Disorganization of the actin cytoskeleton has been previously reported in relation to the CF phenotype. In this work, we aimed to understand this alteration by means of Atomic Force Microscopy and Force Feedback Microscopy investigation of mechanical properties of cystic fibrosis bronchial epithelial (CFBE) cells stably transduced with either wild type (wt-) or F508del-CFTR. We show here that the expression of mutant CFTR causes a decrease in the cell’s apparent Young modulus as compared to the expression of the wt protein. View Full-Text
Keywords: CFTR; AFM; Young modulus CFTR; AFM; Young modulus
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MDPI and ACS Style

Carapeto, A.P.; Vitorino, M.V.; Santos, J.D.; Ramalho, S.S.; Robalo, T.; Rodrigues, M.S.; Farinha, C.M. Mechanical Properties of Human Bronchial Epithelial Cells Expressing Wt- and Mutant CFTR. Int. J. Mol. Sci. 2020, 21, 2916. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082916

AMA Style

Carapeto AP, Vitorino MV, Santos JD, Ramalho SS, Robalo T, Rodrigues MS, Farinha CM. Mechanical Properties of Human Bronchial Epithelial Cells Expressing Wt- and Mutant CFTR. International Journal of Molecular Sciences. 2020; 21(8):2916. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082916

Chicago/Turabian Style

Carapeto, Ana P., Miguel V. Vitorino, João D. Santos, Sofia S. Ramalho, Tiago Robalo, Mário S. Rodrigues, and Carlos M. Farinha 2020. "Mechanical Properties of Human Bronchial Epithelial Cells Expressing Wt- and Mutant CFTR" International Journal of Molecular Sciences 21, no. 8: 2916. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082916

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