Next Article in Journal
Hemokinin-1 Gene Expression Is Upregulated in Trigeminal Ganglia in an Inflammatory Orofacial Pain Model: Potential Role in Peripheral Sensitization
Next Article in Special Issue
Disease-Specific Derangement of Circulating Endocannabinoids and N-Acylethanolamines in Myeloproliferative Neoplasms
Previous Article in Journal
Non-Starch Polysaccharides in Durum Wheat: A Review
Article

Sensitivity of the Fasciae to the Endocannabinoid System: Production of Hyaluronan-Rich Vesicles and Potential Peripheral Effects of Cannabinoids in Fascial Tissue

Department of Neurosciences, Institute of Human Anatomy, University of Padova, Via A. Gabelli 65, 35121 Padova, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(8), 2936; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082936
Received: 30 March 2020 / Revised: 17 April 2020 / Accepted: 20 April 2020 / Published: 22 April 2020
The demonstrated expression of endocannabinoid receptors in myofascial tissue suggested the role of fascia as a source and modulator of pain. Fibroblasts can modulate the production of the various components of the extracellular matrix, according to type of stimuli: physical, mechanical, hormonal, and pharmacological. In this work, fascial fibroblasts were isolated from small samples of human fascia lata of the thigh, collected from three volunteer patients (two men, one woman) during orthopedic surgery. This text demonstrates for the first time that the agonist of cannabinoid receptor 2, HU-308, can lead to in vitro production of hyaluronan-rich vesicles only 3–4 h after treatment, being rapidly released into the extracellular environment. We demonstrated that these vesicles are rich in hyaluronan after Alcian blue and Toluidine blue stainings, immunocytochemistry, and transmission electron microscopy. In addition, incubation with the antagonist AM630 blocked vesicles production by cells, confirming that release of hyaluronan is a cannabinoid-mediated effect. These results may show how fascial cells respond to the endocannabinoid system by regulating and remodeling the formation of the extracellular matrix. This is a first step in our understanding of how therapeutic applications of cannabinoids to treat pain may also have a peripheral effect, altering the biosynthesis of the extracellular matrix in fasciae and, consequently, remodeling the tissue and its properties. View Full-Text
Keywords: fascia; hyaluronan; vesicles; endocannabinoid; agonist; antagonist; inflammation; fibrosis; pain; peripheral effect fascia; hyaluronan; vesicles; endocannabinoid; agonist; antagonist; inflammation; fibrosis; pain; peripheral effect
Show Figures

Figure 1

MDPI and ACS Style

Fede, C.; Pirri, C.; Petrelli, L.; Guidolin, D.; Fan, C.; De Caro, R.; Stecco, C. Sensitivity of the Fasciae to the Endocannabinoid System: Production of Hyaluronan-Rich Vesicles and Potential Peripheral Effects of Cannabinoids in Fascial Tissue. Int. J. Mol. Sci. 2020, 21, 2936. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082936

AMA Style

Fede C, Pirri C, Petrelli L, Guidolin D, Fan C, De Caro R, Stecco C. Sensitivity of the Fasciae to the Endocannabinoid System: Production of Hyaluronan-Rich Vesicles and Potential Peripheral Effects of Cannabinoids in Fascial Tissue. International Journal of Molecular Sciences. 2020; 21(8):2936. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082936

Chicago/Turabian Style

Fede, Caterina, Carmelo Pirri, Lucia Petrelli, Diego Guidolin, Chenglei Fan, Raffaele De Caro, and Carla Stecco. 2020. "Sensitivity of the Fasciae to the Endocannabinoid System: Production of Hyaluronan-Rich Vesicles and Potential Peripheral Effects of Cannabinoids in Fascial Tissue" International Journal of Molecular Sciences 21, no. 8: 2936. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21082936

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop