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Article

Wood Architecture and Composition Are Deeply Remodeled in Frost Sensitive Eucalyptus Overexpressing CBF/DREB1 Transcription Factors

1
Laboratoire de Recherche en Sciences Végétales, Université de Toulouse III, CNRS, UPS, UMR 5546, 31320 Castanet-Tolosan, France
2
Department of Natural Sciences, Hung Vuong University, Nong Trang Ward, Viet Tri City, Phu Tho Province 29000, Vietnam
3
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), University of Pretoria, Pretoria 0002, South Africa
4
Biotechnology and crop protection Department; Northern Mountainous Agriculture and Forestry Science Institute, Phu Tho 29000, Vietnam
5
CMEAB, IFR40 Pôle de Biotechnologie Végétale, 31320 Castanet-Tolosan, France
*
Author to whom correspondence should be addressed.
These two authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(8), 3019; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21083019
Received: 28 March 2020 / Revised: 20 April 2020 / Accepted: 21 April 2020 / Published: 24 April 2020
(This article belongs to the Special Issue Woody Formation and Lignin Biosynthesis in Plants)
Eucalypts are the most planted trees worldwide, but most of them are frost sensitive. Overexpressing transcription factors for CRT-repeat binding factors (CBFs) in transgenic Eucalyptus confer cold resistance both in leaves and stems. While wood plays crucial roles in trees and is affected by environmental cues, its potential role in adaptation to cold stress has been neglected. Here, we addressed this question by investigating the changes occurring in wood in response to the overexpression of two CBFs, taking advantage of available transgenic Eucalyptus lines. We performed histological, biochemical, and transcriptomic analyses on xylem samples. CBF ectopic expression led to a reduction of both primary and secondary growth, and triggered changes in xylem architecture with smaller and more frequent vessels and fibers exhibiting reduced lumens. In addition, lignin content and syringyl/guaiacyl (S/G) ratio increased. Consistently, many genes of the phenylpropanoid and lignin branch pathway were upregulated. Most of the features of xylem remodeling induced by CBF overexpression are reminiscent of those observed after long exposure of Eucalyptus trees to chilling temperatures. Altogether, these results suggest that CBF plays a central role in the cross-talk between response to cold and wood formation and that the remodeling of wood is part of the adaptive strategies to face cold stress. View Full-Text
Keywords: Wood; CBF/DREB1 transcription factors; transgenic Eucalyptus; cold stress; secondary cell walls; lignin; vessels; fibers; wood anatomy Wood; CBF/DREB1 transcription factors; transgenic Eucalyptus; cold stress; secondary cell walls; lignin; vessels; fibers; wood anatomy
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MDPI and ACS Style

Cao, P.B.; Ployet, R.; Nguyen, C.; Dupas, A.; Ladouce, N.; Martinez, Y.; Grima-Pettenati, J.; Marque, C.; Mounet, F.; Teulières, C. Wood Architecture and Composition Are Deeply Remodeled in Frost Sensitive Eucalyptus Overexpressing CBF/DREB1 Transcription Factors. Int. J. Mol. Sci. 2020, 21, 3019. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21083019

AMA Style

Cao PB, Ployet R, Nguyen C, Dupas A, Ladouce N, Martinez Y, Grima-Pettenati J, Marque C, Mounet F, Teulières C. Wood Architecture and Composition Are Deeply Remodeled in Frost Sensitive Eucalyptus Overexpressing CBF/DREB1 Transcription Factors. International Journal of Molecular Sciences. 2020; 21(8):3019. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21083019

Chicago/Turabian Style

Cao, Phi B., Raphaël Ployet, Chien Nguyen, Annabelle Dupas, Nathalie Ladouce, Yves Martinez, Jacqueline Grima-Pettenati, Christiane Marque, Fabien Mounet, and Chantal Teulières. 2020. "Wood Architecture and Composition Are Deeply Remodeled in Frost Sensitive Eucalyptus Overexpressing CBF/DREB1 Transcription Factors" International Journal of Molecular Sciences 21, no. 8: 3019. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms21083019

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