Effect of Amyloid-β Monomers on Lipid Membrane Mechanical Parameters–Potential Implications for Mechanically Driven Neurodegeneration in Alzheimer’s Disease
Abstract
:1. Introduction
2. Results & Discussion
2.1. Effect of Aβ Peptides on Structural Parameters
2.2. Effect of Aβ Peptides on Bending Rigidity Coefficient
2.3. Effect of Aβ Peptides Pressure Wave Propagation
3. Materials and Methods
3.1. Materials
3.2. Preparation of Giant Unilamellar Vesicles (GUVs)
3.3. Confocal Microscopy Imaging and Acquisition
3.4. Flicker-Noise Spectroscopy Analysis
3.5. Molecular Dynamics Simulations
3.6. Determination of Bending Rigidity Coefficient in MD
3.7. Determination of Basic Structural Parameters
3.8. Statistics
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AD | Alzheimer’s disease |
Aβ | amyloid-β |
SA | statistical approach |
AVB | average-based approach |
MD | molecular dynamics |
APL | area per lipid |
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System Description | Membrane Thickness [nm] | APL of Vesicle [Å2] | κ (MD) [J] | κ (Flicker, SA) [J] | κ (Flicker, AVB) [J] |
---|---|---|---|---|---|
POPC [25] | |||||
POPC with 10 m% Aβ-40 | |||||
POPC with 10 m% Aβ-42 | |||||
POPC with 10 m% Aβ-40-TAMRA |
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Drabik, D.; Chodaczek, G.; Kraszewski, S. Effect of Amyloid-β Monomers on Lipid Membrane Mechanical Parameters–Potential Implications for Mechanically Driven Neurodegeneration in Alzheimer’s Disease. Int. J. Mol. Sci. 2021, 22, 18. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010018
Drabik D, Chodaczek G, Kraszewski S. Effect of Amyloid-β Monomers on Lipid Membrane Mechanical Parameters–Potential Implications for Mechanically Driven Neurodegeneration in Alzheimer’s Disease. International Journal of Molecular Sciences. 2021; 22(1):18. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010018
Chicago/Turabian StyleDrabik, Dominik, Grzegorz Chodaczek, and Sebastian Kraszewski. 2021. "Effect of Amyloid-β Monomers on Lipid Membrane Mechanical Parameters–Potential Implications for Mechanically Driven Neurodegeneration in Alzheimer’s Disease" International Journal of Molecular Sciences 22, no. 1: 18. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010018