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Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis

1
Department of Plant Biology and Ecology, College of Life Sciences, Nankai University and Tianjin Key Laboratory of Protein Sciences, Tianjin 300071, China
2
Institute of Biochemistry and Biology, Plant Physiology, University of Potsdam, D-14476 Potsdam-Golm, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(1), 437; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010437
Received: 15 December 2020 / Revised: 29 December 2020 / Accepted: 30 December 2020 / Published: 4 January 2021
(This article belongs to the Section Molecular Plant Sciences)
Plant membrane sterol composition has been reported to affect growth and gravitropism via polar auxin transport and auxin signaling. However, as to whether sterols influence auxin biosynthesis has received little attention. Here, by using the sterol biosynthesis mutant cyclopropylsterol isomerase1-1 (cpi1-1) and sterol application, we reveal that cycloeucalenol, a CPI1 substrate, and sitosterol, an end-product of sterol biosynthesis, antagonistically affect auxin biosynthesis. The short root phenotype of cpi1-1 was associated with a markedly enhanced auxin response in the root tip. Both were neither suppressed by mutations in polar auxin transport (PAT) proteins nor by treatment with a PAT inhibitor and responded to an auxin signaling inhibitor. However, expression of several auxin biosynthesis genes TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1 (TAA1) was upregulated in cpi1-1. Functionally, TAA1 mutation reduced the auxin response in cpi1-1 and partially rescued its short root phenotype. In support of this genetic evidence, application of cycloeucalenol upregulated expression of the auxin responsive reporter DR5:GUS (β-glucuronidase) and of several auxin biosynthesis genes, while sitosterol repressed their expression. Hence, our combined genetic, pharmacological, and sterol application studies reveal a hitherto unexplored sterol-dependent modulation of auxin biosynthesis during Arabidopsis root elongation. View Full-Text
Keywords: Arabidopsis thaliana; auxin; auxin biosynthesis; cycloeucalenol; CPI1; sitosterol; sterol Arabidopsis thaliana; auxin; auxin biosynthesis; cycloeucalenol; CPI1; sitosterol; sterol
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MDPI and ACS Style

Wang, M.; Li, P.; Ma, Y.; Nie, X.; Grebe, M.; Men, S. Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis. Int. J. Mol. Sci. 2021, 22, 437. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010437

AMA Style

Wang M, Li P, Ma Y, Nie X, Grebe M, Men S. Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis. International Journal of Molecular Sciences. 2021; 22(1):437. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010437

Chicago/Turabian Style

Wang, Meng, Panpan Li, Yao Ma, Xiang Nie, Markus Grebe, and Shuzhen Men. 2021. "Membrane Sterol Composition in Arabidopsis thaliana Affects Root Elongation via Auxin Biosynthesis" International Journal of Molecular Sciences 22, no. 1: 437. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010437

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