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Article

Characterization of Urine Stem Cell-Derived Extracellular Vesicles Reveals B Cell Stimulating Cargo

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Health and Biomedical Innovation, Clinical and Health Sciences, University of South Australia, Adelaide, SA 5000, Australia
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Department of Medical Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria 21568, Egypt
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Centre of Excellence for Research in Regenerative Medicine Applications (CERRMA), Faculty of Medicine, Alexandria University, Alexandria 21568, Egypt
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Department of Molecular and Cellular Biology, School of Biological Sciences, University of Adelaide, Adelaide, SA 5000, Australia
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Centre for Orthopaedic and Trauma Research, University of Adelaide, Adelaide, SA 5000, Australia
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Department of Renal Medicine, Royal Adelaide Hospital, Adelaide, SA 5000, Australia
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School of Medicine, University of Adelaide, Adelaide, SA 5000, Australia
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Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2021, 22(1), 459; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010459
Received: 20 December 2020 / Revised: 31 December 2020 / Accepted: 1 January 2021 / Published: 5 January 2021
(This article belongs to the Special Issue Interaction of Nanomaterials with the Immune System)
Elucidation of the biological functions of extracellular vesicles (EVs) and their potential roles in physiological and pathological processes is an expanding field of research. In this study, we characterized USC–derived EVs and studied their capacity to modulate the human immune response in vitro. We found that the USC–derived EVs are a heterogeneous population, ranging in size from that of micro–vesicles (150 nm–1 μm) down to that of exosomes (60–150 nm). Regarding their immunomodulatory functions, we found that upon isolation, the EVs (60–150 nm) induced B cell proliferation and IgM antibody secretion. Analysis of the EV contents unexpectedly revealed the presence of BAFF, APRIL, IL–6, and CD40L, all known to play a central role in B cell stimulation, differentiation, and humoral immunity. In regard to their effect on T cell functions, they resembled the function of mesenchymal stem cell (MSC)–derived EVs previously described, suppressing T cell response to activation. The finding that USC–derived EVs transport a potent bioactive cargo opens the door to a novel therapeutic avenue for boosting B cell responses in immunodeficiency or cancer. View Full-Text
Keywords: exosomes; B cells; BAFF; CD40L; mesenchymal stem cells; urine stem cells; extracellular vesicles exosomes; B cells; BAFF; CD40L; mesenchymal stem cells; urine stem cells; extracellular vesicles
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MDPI and ACS Style

Zidan, A.A.; Al-Hawwas, M.; Perkins, G.B.; Mourad, G.M.; Stapledon, C.J.M.; Bobrovskaya, L.; Zhou, X.-F.; Hurtado, P.R. Characterization of Urine Stem Cell-Derived Extracellular Vesicles Reveals B Cell Stimulating Cargo. Int. J. Mol. Sci. 2021, 22, 459. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010459

AMA Style

Zidan AA, Al-Hawwas M, Perkins GB, Mourad GM, Stapledon CJM, Bobrovskaya L, Zhou X-F, Hurtado PR. Characterization of Urine Stem Cell-Derived Extracellular Vesicles Reveals B Cell Stimulating Cargo. International Journal of Molecular Sciences. 2021; 22(1):459. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010459

Chicago/Turabian Style

Zidan, Asmaa A., Mohammed Al-Hawwas, Griffith B. Perkins, Ghada M. Mourad, Catherine J.M. Stapledon, Larisa Bobrovskaya, Xin-Fu Zhou, and Plinio R. Hurtado 2021. "Characterization of Urine Stem Cell-Derived Extracellular Vesicles Reveals B Cell Stimulating Cargo" International Journal of Molecular Sciences 22, no. 1: 459. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010459

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