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Article

Deficiency in Tissue Non-Specific Alkaline Phosphatase Leads to Steatohepatitis in Mice Fed a High Fat Diet Similar to That Produced by a Methionine and Choline Deficient Diet

1
Department of Pharmacology, CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.GRANADA, University of Granada, 18071 Granada, Spain
2
Department of Biochemistry and Molecular Biology 2, CIBERehd, School of Pharmacy, Instituto de Investigación Biosanitaria ibs.GRANADA, Instituto de Nutrición y Tecnología de los Alimentos José Mataix, University of Granada, 18071 Granada, Spain
*
Author to whom correspondence should be addressed.
Both authors contributed equally to this work.
Int. J. Mol. Sci. 2021, 22(1), 51; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010051
Received: 27 November 2020 / Revised: 18 December 2020 / Accepted: 21 December 2020 / Published: 23 December 2020
The liver expresses tissue-nonspecific alkaline phosphatase (TNAP), which may participate in the defense against bacterial components, in cell regulation as part of the purinome or in bile secretion, among other roles. We aimed to study the role of TNAP in the development of hepatosteatosis. TNAP+/− haplodeficient and wild type (WT) mice were fed a control diet (containing 10% fat w/w) or the same diet deficient in methionine and choline (MCD diet). The MCD diet induced substantial weight loss together with hepatic steatosis and increased alanine aminotransferase (ALT) plasma levels, but no differences in IL-6, TNF, insulin or resistin. There were no substantial differences between TNAP+/− and WT mice fed the MCD diet. In turn, TNAP+/− mice receiving the control diet presented hepatic steatosis with alterations in metabolic parameters very similar to those induced by the MCD diet. Nevertheless, no weight loss, increased ALT plasma levels or hypoglycemia were observed. These mice also presented increased levels of liver TNF and systemic resistin and glucagon compared to WT mice. The phenotype of TNAP+/− mice fed a standard diet was normal. In conclusion, TNAP haplodeficiency induces steatosis comparable to that produced by a MCD diet when fed a control diet. View Full-Text
Keywords: methionine and choline diet; metabolic syndrome; biliary acids; tissue non-specific alkaline phosphatase; steatohepatitis methionine and choline diet; metabolic syndrome; biliary acids; tissue non-specific alkaline phosphatase; steatohepatitis
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MDPI and ACS Style

Gámez-Belmonte, R.; Tena-Garitaonaindia, M.; Hernández-Chirlaque, C.; Córdova, S.; Ceacero-Heras, D.; de Medina, F.S.; Martínez-Augustin, O. Deficiency in Tissue Non-Specific Alkaline Phosphatase Leads to Steatohepatitis in Mice Fed a High Fat Diet Similar to That Produced by a Methionine and Choline Deficient Diet. Int. J. Mol. Sci. 2021, 22, 51. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010051

AMA Style

Gámez-Belmonte R, Tena-Garitaonaindia M, Hernández-Chirlaque C, Córdova S, Ceacero-Heras D, de Medina FS, Martínez-Augustin O. Deficiency in Tissue Non-Specific Alkaline Phosphatase Leads to Steatohepatitis in Mice Fed a High Fat Diet Similar to That Produced by a Methionine and Choline Deficient Diet. International Journal of Molecular Sciences. 2021; 22(1):51. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010051

Chicago/Turabian Style

Gámez-Belmonte, Reyes, Mireia Tena-Garitaonaindia, Cristina Hernández-Chirlaque, Samir Córdova, Diego Ceacero-Heras, Fermín S. de Medina, and Olga Martínez-Augustin. 2021. "Deficiency in Tissue Non-Specific Alkaline Phosphatase Leads to Steatohepatitis in Mice Fed a High Fat Diet Similar to That Produced by a Methionine and Choline Deficient Diet" International Journal of Molecular Sciences 22, no. 1: 51. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22010051

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