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Article

A cis-Acting Mutation in the PxABCG1 Promoter Is Associated with Cry1Ac Resistance in Plutella xylostella (L.)

1
Longping Branch, Graduate School of Hunan University, Changsha 410125, China
2
Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China
3
Department of Entomology, University of Kentucky, Lexington, KY 40546-0091, USA
4
School of Life Sciences, University of Sussex, Brighton BN1 9QG, UK
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editors: Alexander Tonevitsky and Diana Maltseva
Int. J. Mol. Sci. 2021, 22(11), 6106; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22116106
Received: 3 May 2021 / Revised: 2 June 2021 / Accepted: 3 June 2021 / Published: 5 June 2021
The molecular mechanisms of insect resistance to Cry toxins generated from the bacterium Bacillus thuringiensis (Bt) urgently need to be elucidated to enable the improvement and sustainability of Bt-based products. Although downregulation of the expression of midgut receptor genes is a pivotal mechanism of insect resistance to Bt Cry toxins, the underlying transcriptional regulation of these genes remains elusive. Herein, we unraveled the regulatory mechanism of the downregulation of the ABC transporter gene PxABCG1 (also called Pxwhite), a functional midgut receptor of the Bt Cry1Ac toxin in Plutella xylostella. The PxABCG1 promoters of Cry1Ac-susceptible and Cry1Ac-resistant strains were cloned and analyzed, and they showed clear differences in activity. Subsequently, a dual-luciferase reporter assay, a yeast one-hybrid (Y1H) assay, and RNA interference (RNAi) experiments demonstrated that a cis-mutation in a binding site of the Hox transcription factor Antennapedia (Antp) decreased the promoter activity of the resistant strain and eliminated the binding and regulation of Antp, thereby enhancing the resistance of P. xylostella to the Cry1Ac toxin. These results advance our knowledge of the roles of cis- and trans-regulatory variations in the regulation of midgut Cry receptor genes and the evolution of Bt resistance, contributing to a more complete understanding of the Bt resistance mechanism. View Full-Text
Keywords: Bacillus thuringiensis; Plutella xylostella; cis-mutation; Antp; ABCG1; Cry1Ac resistance Bacillus thuringiensis; Plutella xylostella; cis-mutation; Antp; ABCG1; Cry1Ac resistance
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MDPI and ACS Style

Qin, J.; Ye, F.; Xu, L.; Zhou, X.; Crickmore, N.; Zhou, X.; Zhang, Y.; Guo, Z. A cis-Acting Mutation in the PxABCG1 Promoter Is Associated with Cry1Ac Resistance in Plutella xylostella (L.). Int. J. Mol. Sci. 2021, 22, 6106. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22116106

AMA Style

Qin J, Ye F, Xu L, Zhou X, Crickmore N, Zhou X, Zhang Y, Guo Z. A cis-Acting Mutation in the PxABCG1 Promoter Is Associated with Cry1Ac Resistance in Plutella xylostella (L.). International Journal of Molecular Sciences. 2021; 22(11):6106. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22116106

Chicago/Turabian Style

Qin, Jianying, Fan Ye, Linzheng Xu, Xuguo Zhou, Neil Crickmore, Xiaomao Zhou, Youjun Zhang, and Zhaojiang Guo. 2021. "A cis-Acting Mutation in the PxABCG1 Promoter Is Associated with Cry1Ac Resistance in Plutella xylostella (L.)" International Journal of Molecular Sciences 22, no. 11: 6106. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22116106

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