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Article

Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns of IL6 and ADIPOQ Genes and Their Associated Epigenetic Modifications in Differentiating Human Visceral Adipocytes

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Department of Medical Biochemistry, Medical University of Lodz, 92-215 Lodz, Poland
2
Department of Molecular Biophysics, University of Lodz, 90-236 Lodz, Poland
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Central Teaching Hospital of the Medical University of Lodz, 92-213 Lodz, Poland
4
Department of Nucleic Acids Biochemistry, Medical University of Lodz, 92-213 Lodz, Poland
*
Authors to whom correspondence should be addressed.
Academic Editor: Grażyna Nowicka
Int. J. Mol. Sci. 2021, 22(13), 6964; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22136964
Received: 20 May 2021 / Revised: 22 June 2021 / Accepted: 23 June 2021 / Published: 28 June 2021
(This article belongs to the Special Issue Obesity, Genes, and Obesity-Related Disorders)
Adipokines secreted by hypertrophic visceral adipose tissue (VAT) instigate low-grade inflammation, followed by hyperglycemia (HG)-related metabolic disorders. The latter may develop with the participation of epigenetic modifications. Our aim was to assess how HG influences selected epigenetic modifications and the expression of interleukin 6 (IL-6) and adiponectin (APN; gene symbol ADIPOQ) during the adipogenesis of human visceral preadipocytes (HPA-v). Adipocytes (Ads) were chronically or transiently HG-treated during three stages of adipogenesis (proliferation, differentiation, maturation). We measured adipokine mRNA, protein, proven or predicted microRNA expression (RT-qPCR and ELISA), and enrichment of H3K9/14ac, H3K4me3, and H3K9me3 at gene promoter regions (chromatin immunoprecipitation). In chronic HG, we detected different expression patterns of the studied adipokines at the mRNA and protein levels. Chronic and transient HG-induced changes in miRNA (miR-26a-5p, miR-26b-5p, let-7d-5p, let-7e-5p, miR-365a-3p, miR-146a-5p) were mostly convergent to altered IL-6 transcription. Alterations in histone marks at the IL6 promoter were also in agreement with IL-6 mRNA. The open chromatin marks at the ADIPOQ promoter mostly reflected the APN transcription during NG adipogenesis, while, in the differentiation stage, HG-induced changes in all studied marks were in line with APN mRNA levels. In summary, HG dysregulated adipokine expression, promoting inflammation. Epigenetic changes coexisted with altered expression of adipokines, especially for IL-6; therefore, epigenetic marks induced by transient HG may act as epi-memory in Ads. Such changes in the epigenome and expression of adipokines could be instrumental in the development of inflammation and metabolic deregulation of VAT. View Full-Text
Keywords: visceral preadipocytes; adipocytes; interleukin 6; adiponectin; adipogenesis; histone modifications; microRNA; inflammation; hyperglycemia; diabetes visceral preadipocytes; adipocytes; interleukin 6; adiponectin; adipogenesis; histone modifications; microRNA; inflammation; hyperglycemia; diabetes
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MDPI and ACS Style

Wróblewski, A.; Strycharz, J.; Świderska, E.; Balcerczyk, A.; Szemraj, J.; Drzewoski, J.; Śliwińska, A. Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns of IL6 and ADIPOQ Genes and Their Associated Epigenetic Modifications in Differentiating Human Visceral Adipocytes. Int. J. Mol. Sci. 2021, 22, 6964. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22136964

AMA Style

Wróblewski A, Strycharz J, Świderska E, Balcerczyk A, Szemraj J, Drzewoski J, Śliwińska A. Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns of IL6 and ADIPOQ Genes and Their Associated Epigenetic Modifications in Differentiating Human Visceral Adipocytes. International Journal of Molecular Sciences. 2021; 22(13):6964. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22136964

Chicago/Turabian Style

Wróblewski, Adam, Justyna Strycharz, Ewa Świderska, Aneta Balcerczyk, Janusz Szemraj, Józef Drzewoski, and Agnieszka Śliwińska. 2021. "Chronic and Transient Hyperglycemia Induces Changes in the Expression Patterns of IL6 and ADIPOQ Genes and Their Associated Epigenetic Modifications in Differentiating Human Visceral Adipocytes" International Journal of Molecular Sciences 22, no. 13: 6964. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22136964

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