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Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts

1
Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, China
2
Center of Excellence in Genomics & Systems Biology, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad 502324, India
3
Oil Research Institute of Guizhou Province, Guizhou Academy of Agricultural Science, Guiyang 550006, China
4
State Agricultural Biotechnology Centre, Centre for Crop and Food Innovation, Murdoch University, Murdoch 6150, Australia
*
Author to whom correspondence should be addressed.
Academic Editor: Bartolome Sabater
Int. J. Mol. Sci. 2021, 22(14), 7266; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147266
Received: 20 May 2021 / Revised: 2 July 2021 / Accepted: 3 July 2021 / Published: 6 July 2021
(This article belongs to the Section Molecular Plant Sciences)
Sucrose content is a crucial indicator of quality and flavor in peanut seed, and there is a lack of clarity on the molecular basis of sucrose metabolism in peanut seed. In this context, we performed a comprehensive comparative transcriptome study on the samples collected at seven seed development stages between a high-sucrose content variety (ICG 12625) and a low-sucrose content variety (Zhonghua 10). The transcriptome analysis identified a total of 8334 genes exhibiting significantly different abundances between the high- and low-sucrose varieties. We identified 28 differentially expressed genes (DEGs) involved in sucrose metabolism in peanut and 12 of these encoded sugars will eventually be exported transporters (SWEETs). The remaining 16 genes encoded enzymes, such as cell wall invertase (CWIN), vacuolar invertase (VIN), cytoplasmic invertase (CIN), cytosolic fructose-bisphosphate aldolase (FBA), cytosolic fructose-1,6-bisphosphate phosphatase (FBP), sucrose synthase (SUS), cytosolic phosphoglucose isomerase (PGI), hexokinase (HK), and sucrose-phosphate phosphatase (SPP). The weighted gene co-expression network analysis (WGCNA) identified seven genes encoding key enzymes (CIN, FBA, FBP, HK, and SPP), three SWEET genes, and 90 transcription factors (TFs) showing a high correlation with sucrose content. Furthermore, upon validation, six of these genes were successfully verified as exhibiting higher expression in high-sucrose recombinant inbred lines (RILs). Our study suggested the key roles of the high expression of SWEETs and enzymes in sucrose synthesis making the genotype ICG 12625 sucrose-rich. This study also provided insights into the molecular basis of sucrose metabolism during seed development and facilitated exploring key candidate genes and molecular breeding for sucrose content in peanuts. View Full-Text
Keywords: transcriptional regulation; sucrose metabolism; RNA-based sequencing (RNA-seq); development; peanut seed transcriptional regulation; sucrose metabolism; RNA-based sequencing (RNA-seq); development; peanut seed
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MDPI and ACS Style

Li, W.; Huang, L.; Liu, N.; Pandey, M.K.; Chen, Y.; Cheng, L.; Guo, J.; Yu, B.; Luo, H.; Zhou, X.; Huai, D.; Chen, W.; Yan, L.; Wang, X.; Lei, Y.; Varshney, R.K.; Liao, B.; Jiang, H. Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts. Int. J. Mol. Sci. 2021, 22, 7266. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147266

AMA Style

Li W, Huang L, Liu N, Pandey MK, Chen Y, Cheng L, Guo J, Yu B, Luo H, Zhou X, Huai D, Chen W, Yan L, Wang X, Lei Y, Varshney RK, Liao B, Jiang H. Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts. International Journal of Molecular Sciences. 2021; 22(14):7266. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147266

Chicago/Turabian Style

Li, Weitao, Li Huang, Nian Liu, Manish K. Pandey, Yuning Chen, Liangqiang Cheng, Jianbin Guo, Bolun Yu, Huaiyong Luo, Xiaojing Zhou, Dongxin Huai, Weigang Chen, Liying Yan, Xin Wang, Yong Lei, Rajeev K. Varshney, Boshou Liao, and Huifang Jiang. 2021. "Key Regulators of Sucrose Metabolism Identified through Comprehensive Comparative Transcriptome Analysis in Peanuts" International Journal of Molecular Sciences 22, no. 14: 7266. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147266

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