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Article

A Complex Network of Sigma Factors and sRNA StsR Regulates Stress Responses in R. sphaeroides

Institute of Microbiology and Molecular Biology, Justus-Liebig University of Giessen, IFZ, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany
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Academic Editor: Jill Zeilstra-Ryalls
Int. J. Mol. Sci. 2021, 22(14), 7557; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147557
Received: 11 June 2021 / Revised: 1 July 2021 / Accepted: 9 July 2021 / Published: 14 July 2021
(This article belongs to the Special Issue Molecular Biology of Phototrophic Bacterial)
Adaptation of bacteria to a changing environment is often accompanied by remodeling of the transcriptome. In the facultative phototroph Rhodobacter sphaeroides the alternative sigma factors RpoE, RpoHI and RpoHII play an important role in a variety of stress responses, including heat, oxidative stress and nutrient limitation. Photooxidative stress caused by the simultaneous presence of chlorophylls, light and oxygen is a special challenge for phototrophic organisms. Like alternative sigma factors, several non-coding sRNAs have important roles in the defense against photooxidative stress. RNAseq-based transcriptome data pointed to an influence of the stationary phase-induced StsR sRNA on levels of mRNAs and sRNAs with a role in the photooxidative stress response. Furthermore, StsR also affects expression of photosynthesis genes and of genes for regulators of photosynthesis genes. In vivo and in vitro interaction studies revealed that StsR, that is under control of the RpoHI and RpoHII sigma factors, targets rpoE mRNA and affects its abundance by altering its stability. RpoE regulates expression of the rpoHII gene and, consequently, expression of stsR. These data provide new insights into a complex regulatory network of protein regulators and sRNAs involved in defense against photooxidative stress and the regulation of photosynthesis genes. View Full-Text
Keywords: anoxygenic photosynthesis; photooxidative stress; alternative sigma factor; sRNAs; transcriptome; regulatory networks anoxygenic photosynthesis; photooxidative stress; alternative sigma factor; sRNAs; transcriptome; regulatory networks
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MDPI and ACS Style

Eisenhardt, K.M.H.; Remes, B.; Grützner, J.; Spanka, D.-T.; Jäger, A.; Klug, G. A Complex Network of Sigma Factors and sRNA StsR Regulates Stress Responses in R. sphaeroides. Int. J. Mol. Sci. 2021, 22, 7557. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147557

AMA Style

Eisenhardt KMH, Remes B, Grützner J, Spanka D-T, Jäger A, Klug G. A Complex Network of Sigma Factors and sRNA StsR Regulates Stress Responses in R. sphaeroides. International Journal of Molecular Sciences. 2021; 22(14):7557. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147557

Chicago/Turabian Style

Eisenhardt, Katrin M.H., Bernhardt Remes, Julian Grützner, Daniel-Timon Spanka, Andreas Jäger, and Gabriele Klug. 2021. "A Complex Network of Sigma Factors and sRNA StsR Regulates Stress Responses in R. sphaeroides" International Journal of Molecular Sciences 22, no. 14: 7557. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22147557

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