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Intrauterine Nitric Oxide Deficiency Weakens Differentiation of Vascular Smooth Muscle in Newborn Rats

1
Department of Human and Animal Physiology, Faculty of Biology, M.V. Lomonosov Moscow State University, 119234 Moscow, Russia
2
Laboratory of Exercise Physiology, State Research Center of the Russian Federation-Institute for Biomedical Problems, Russian Academy of Sciences, 123007 Moscow, Russia
3
Laboratory for the Study of Information Processes at the Cellular and Molecular Levels, Institute for Information Transmission Problems, Russian Academy of Sciences, 119333 Moscow, Russia
4
Department of Physiology, Russian National Research Medical University, 117513 Moscow, Russia
*
Author to whom correspondence should be addressed.
Academic Editor: Joško Osredkar
Int. J. Mol. Sci. 2021, 22(15), 8003; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22158003
Received: 6 July 2021 / Revised: 22 July 2021 / Accepted: 24 July 2021 / Published: 27 July 2021
Nitric oxide (NO) deficiency during pregnancy is a key reason for preeclampsia development. Besides its important vasomotor role, NO is shown to regulate the cell transcriptome. However, the role of NO in transcriptional regulation of developing smooth muscle has never been studied before. We hypothesized that in early ontogeny, NO is important for the regulation of arterial smooth muscle-specific genes expression. Pregnant rats consumed NO-synthase inhibitor L-NAME (500 mg/L in drinking water) from gestational day 10 till delivery, which led to an increase in blood pressure, a key manifestation of preeclampsia. L-NAME reduced blood concentrations of NO metabolites in dams and their newborn pups, as well as relaxations of pup aortic rings to acetylcholine. Using qPCR, we demonstrated reduced abundances of the smooth muscle-specific myosin heavy chain isoform, α-actin, SM22α, and L-type Ca2+-channel mRNAs in the aorta of newborn pups from the L-NAME group compared to control pups. To conclude, the intrauterine NO deficiency weakens gene expression specific for a contractile phenotype of arterial smooth muscle in newborn offspring. View Full-Text
Keywords: nitric oxide; preeclampsia; prenatal ontogenesis; vascular smooth muscle cells; synthetic phenotype; contractile phenotype nitric oxide; preeclampsia; prenatal ontogenesis; vascular smooth muscle cells; synthetic phenotype; contractile phenotype
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MDPI and ACS Style

Shvetsova, A.A.; Borzykh, A.A.; Selivanova, E.K.; Kiryukhina, O.O.; Gaynullina, D.K.; Tarasova, O.S. Intrauterine Nitric Oxide Deficiency Weakens Differentiation of Vascular Smooth Muscle in Newborn Rats. Int. J. Mol. Sci. 2021, 22, 8003. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22158003

AMA Style

Shvetsova AA, Borzykh AA, Selivanova EK, Kiryukhina OO, Gaynullina DK, Tarasova OS. Intrauterine Nitric Oxide Deficiency Weakens Differentiation of Vascular Smooth Muscle in Newborn Rats. International Journal of Molecular Sciences. 2021; 22(15):8003. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22158003

Chicago/Turabian Style

Shvetsova, Anastasia A., Anna A. Borzykh, Ekaterina K. Selivanova, Oxana O. Kiryukhina, Dina K. Gaynullina, and Olga S. Tarasova 2021. "Intrauterine Nitric Oxide Deficiency Weakens Differentiation of Vascular Smooth Muscle in Newborn Rats" International Journal of Molecular Sciences 22, no. 15: 8003. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22158003

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