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Article

Comparison of Histone H3K4me3 between IVF and ICSI Technologies and between Boy and Girl Offspring

1
Department of Obstetrics and Gynecology, University Hospital, LMU Munich, 80337 Munich, Germany
2
Department of Obstetrics and Gynecology, University Hospital Augsburg, 86156 Augsburg, Germany
*
Author to whom correspondence should be addressed.
Academic Editor: Maurizio Battino
Int. J. Mol. Sci. 2021, 22(16), 8574; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168574
Received: 23 July 2021 / Revised: 3 August 2021 / Accepted: 6 August 2021 / Published: 9 August 2021
(This article belongs to the Special Issue Reproductive Immunology: Cellular and Molecular Biology 2.0)
Epigenetics play a vital role in early embryo development. Offspring conceived via assisted reproductive technologies (ARTs) have a three times higher risk of epigenetic diseases than naturally conceived children. However, investigations into ART-associated placental histone modifications or sex-stratified analyses of ART-associated histone modifications remain limited. In the current study, we carried out immunohistochemistry, chip-sequence analysis, and a series of in vitro experiments. Our results demonstrated that placentas from intra-cytoplasmic sperm injection (ICSI), but not in vitro fertilization (IVF), showed global tri-methylated-histone-H3-lysine-4 (H3K4me3) alteration compared to those from natural conception. However, for acetylated-histone-H3-lysine-9 (H3K9ac) and acetylated-histone-H3-lysine-27 (H3K27ac), no significant differences between groups could be found. Further, sex -stratified analysis found that, compared with the same-gender newborn cord blood mononuclear cell (CBMC) from natural conceptions, CBMC from ICSI-boys presented more genes with differentially enriched H3K4me3 (n = 198) than those from ICSI-girls (n = 79), IVF-girls (n = 5), and IVF-boys (n = 2). We also found that varying oxygen conditions, RNA polymerase II subunit A (Polr2A), and lysine demethylase 5A (KDM5A) regulated H3K4me3. These findings revealed a difference between IVF and ICSI and a difference between boys and girls in H3K4me3 modification, providing greater insight into ART-associated epigenetic alteration. View Full-Text
Keywords: assisted reproductive technologies; ICSI; IVF; H3K4me3; placenta; umbilical cord blood; imprint; oxygen; Polr2A; KDM5A assisted reproductive technologies; ICSI; IVF; H3K4me3; placenta; umbilical cord blood; imprint; oxygen; Polr2A; KDM5A
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MDPI and ACS Style

Yang, H.; Ma, Z.; Peng, L.; Kuhn, C.; Rahmeh, M.; Mahner, S.; Jeschke, U.; von Schönfeldt, V. Comparison of Histone H3K4me3 between IVF and ICSI Technologies and between Boy and Girl Offspring. Int. J. Mol. Sci. 2021, 22, 8574. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168574

AMA Style

Yang H, Ma Z, Peng L, Kuhn C, Rahmeh M, Mahner S, Jeschke U, von Schönfeldt V. Comparison of Histone H3K4me3 between IVF and ICSI Technologies and between Boy and Girl Offspring. International Journal of Molecular Sciences. 2021; 22(16):8574. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168574

Chicago/Turabian Style

Yang, Huixia, Zhi Ma, Lin Peng, Christina Kuhn, Martina Rahmeh, Sven Mahner, Udo Jeschke, and Viktoria von Schönfeldt. 2021. "Comparison of Histone H3K4me3 between IVF and ICSI Technologies and between Boy and Girl Offspring" International Journal of Molecular Sciences 22, no. 16: 8574. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22168574

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