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Article

Protein Adsorption onto Modified Porous Silica by Single and Binary Human Serum Protein Solutions

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Centro de Tecnologia, Grupo de Pesquisa em Separações por Adsorção—GPSA—Departamento de Engenharia Química, Campus do Pici, Bl. 709, Universidade Federal do Ceará, Fortaleza 60455-760, CE, Brazil
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Departamento de Química Inorgánica, Cristalografía y Mineralogía, Facultad de Ciencias, Campus de Teatinos, Universidad de Málaga, 29071 Malaga, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Andrea Salis
Int. J. Mol. Sci. 2021, 22(17), 9164; https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179164
Received: 26 July 2021 / Revised: 16 August 2021 / Accepted: 21 August 2021 / Published: 25 August 2021
Typical porous silica (SBA-15) has been modified with pore expander agent (1,3,5-trimethylbenzene) and fluoride-species to diminish the length of the channels to obtain materials with different textural properties, varying the Si/Zr molar ratio between 20 and 5. These porous materials were characterized by X-ray Diffraction (XRD), N2 adsorption/desorption isotherms at −196 °C and X-ray Photoelectron Spectroscopy (XPS), obtaining adsorbent with a surface area between 420–337 m2 g−1 and an average pore diameter with a maximum between 20–25 nm. These materials were studied in the adsorption of human blood serum proteins (human serum albumin—HSA and immunoglobulin G—IgG). Generally, the incorporation of small proportions was favorable for proteins adsorption. The adsorption data revealed that the maximum adsorption capacity was reached close to the pI. The batch purification experiments in binary human serum solutions showed that Si sample has considerable adsorption for IgG while HSA adsorption is relatively low, so it is possible its separation. View Full-Text
Keywords: purification; adsorption isotherm; human serum: porous silica; SBA-15; mesocellular foam purification; adsorption isotherm; human serum: porous silica; SBA-15; mesocellular foam
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MDPI and ACS Style

Gondim, D.R.; Cecilia, J.A.; Rodrigues, T.N.B.; Vilarrasa-García, E.; Rodríguez-Castellón, E.; Azevedo, D.C.S.; Silva, I.J., Jr. Protein Adsorption onto Modified Porous Silica by Single and Binary Human Serum Protein Solutions. Int. J. Mol. Sci. 2021, 22, 9164. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179164

AMA Style

Gondim DR, Cecilia JA, Rodrigues TNB, Vilarrasa-García E, Rodríguez-Castellón E, Azevedo DCS, Silva IJ Jr.. Protein Adsorption onto Modified Porous Silica by Single and Binary Human Serum Protein Solutions. International Journal of Molecular Sciences. 2021; 22(17):9164. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179164

Chicago/Turabian Style

Gondim, Diego R., Juan A. Cecilia, Thaina N.B. Rodrigues, Enrique Vilarrasa-García, Enrique Rodríguez-Castellón, Diana C.S. Azevedo, and Ivanildo J. Silva Jr. 2021. "Protein Adsorption onto Modified Porous Silica by Single and Binary Human Serum Protein Solutions" International Journal of Molecular Sciences 22, no. 17: 9164. https://0-doi-org.brum.beds.ac.uk/10.3390/ijms22179164

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